hH-Rev107, a class II tumor suppressor gene, is expressed by post-meiotic testicular germ cells and CIS cells but not by human testicular germ cell tumors
| Autor: | Frédérique Bulle, Brigitte Solhonne, Ewa Rajpert-De Meyts, Chloé C. Féral, S. Siegrist, Georges Guellaen, Marie-Geneviève Mattei, Mounia Chami |
|---|---|
| Přispěvatelé: | Régulation des gènes et signalisation cellulaire, Institut National de la Santé et de la Recherche Médicale (INSERM), Département de génétique médicale [Hôpital de la Timone - APHM], Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Growth and Reproduction [Rigshospitalet], Rigshospitalet [Copenhagen], Copenhagen University Hospital-Copenhagen University Hospital, INSERM, Danish Cancer Society, Maillochon, Franck |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Male
Cancer Research Cell MESH: Amino Acid Sequence MESH: Base Sequence MESH: Down-Regulation Testis Gene expression Tissue Distribution MESH: Proteins tumor suppressor gene Intracellular Signaling Peptides and Proteins Chromosome Mapping Neoplasms Germ Cell and Embryonal Spermatozoa Meiosis medicine.anatomical_structure MESH: Protein Biosynthesis Phospholipases A2 Calcium-Independent MESH: Neoplasms Germ Cell and Embryonal testicular germ cell tumors Tumor suppressor gene Molecular Sequence Data Down-Regulation [SDV.CAN]Life Sciences [q-bio]/Cancer In situ hybridization Biology Article [SDV.CAN] Life Sciences [q-bio]/Cancer Complementary DNA [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Genetics medicine Humans MESH: Blotting Northern [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Amino Acid Sequence Northern blot Molecular Biology Gene MESH: Humans MESH: Molecular Sequence Data Base Sequence MESH: Research Support Chromosomes Human Pair 11 Tumor Suppressor Proteins Proteins carcinoma in situ Blotting Northern medicine.disease Molecular biology spermatogenesis MESH: Male MESH: Meiosis Protein Biosynthesis hH-Rev107 Germ cell tumors MESH: Chromosomes Human Pair 11 MESH: Chromosome Mapping |
| Zdroj: | Oncogene Oncogene, Nature Publishing Group, 2001, 20 (37), pp.5155-63. ⟨10.1038/sj/onc/1204658⟩ |
| ISSN: | 0950-9232 1476-5594 |
| DOI: | 10.1038/sj/onc/1204658⟩ |
| Popis: | By systematic analysis of a human testis library, we have isolated the hH-Rev107-3 cDNA, identical to hH-Rev107-1 cDNA, which was previously described as a class II tumor suppressor gene (Husmann et al., 1998). In this study, two transcripts (1 and 0,8 kb) were detected by Northern blot in all human tissues, excepted in thymus. The strongest expression was found in testis, skeletal muscle and heart. These two mRNA are probably transcribed from only one gene that we mapped to the q12–q13 region of the chromosome 11. In human testis, hH-Rev107 gene expression was localized, by in situ hybridization, within the round spermatids. To investigate a possible role for hH-Rev107 protein in testicular malignant growth, we examined the expression of this gene in germ cell tumors. A strong hH-Rev107 gene expression was observed in normal testis as well as in samples with preinvasive carcinoma in situ but was completely absent in overt tumors, both seminomas and non-seminomas. By in situ hybridization, CIS was found hH-Rev107 positive and tumor negative. A semi-quantitative assessment of hH-Rev107 mRNA level in testicular germ cell tumors, by RT-PCR, exhibited a 9-fold decrease in the gene expression. No gross structural aberrations of hH-Rev107 gene were detected in these human primary tumors. The results suggest that down-regulation of hH-Rev107 may be associated with invasive progression of testicular germ cell tumors. |
| Databáze: | OpenAIRE |
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