Tracing Surrogates for Enteric Pathogens Inoculated on Hide through the Beef Harvesting Process
| Autor: | Mariana Villarreal-Silva, James S. Dickson, Jeffrey W. Savell, Irfan Ilhak, Alejandro Castillo, Lisa M. Lucia, Kerri B. Gehring, Daniel P. Genho |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Veterinary medicine Salmonella Meat Microorganism 030106 microbiology Colony Count Microbial Food Contamination Biology Escherichia coli O157 medicine.disease_cause Microbiology 03 medical and health sciences fluids and secretions medicine Animals Pathogen Inoculation technology industry and agriculture 0402 animal and dairy science food and beverages 04 agricultural and veterinary sciences Contamination Antimicrobial 040201 dairy & animal science Red Meat Food Microbiology Postharvest Cattle Evisceration (autotomy) Abattoirs Food Science |
| Zdroj: | Journal of Food Protection. 79:1860-1867 |
| ISSN: | 0362-028X |
| DOI: | 10.4315/0362-028x.jfp-15-481 |
| Popis: | Multiple antimicrobial interventions have been validated for use during the beef postharvesting process. However, little has been done to determine the impact of the postharvest environment on pathogen contamination. In this study, surrogate microorganisms were used to simulate pathogen cross-contamination through the postharvest environment at three different abattoirs. At each abattoir, the brisket areas of 13 hide-on carcasses were inoculated after stunning, with a gelatin slurry containing a cocktail (~7 log CFU/ml) of fluorescent Escherichia coli biotype I. These microorganisms are approved as surrogates for E. coli O157:H7 and Salmonella. From these carcasses, samples (300 cm2) were taken at different stages during the harvesting process: after hide opening, prior to evisceration, after evisceration, after splitting, and after final intervention. The carcass (noninoculated) immediately following (adjacent to) each hide-inoculated carcass was also tested to determine cross-contamination. Env... |
| Databáze: | OpenAIRE |
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