Purification and Characterization of the Bovine Pituitary Luteinizing Hormone Releasing Hormone Mr 60000 Binding Protein
| Autor: | Jesper Christiansen, Gunnar Houen, Jørgen Glerup, Markus Neuburger, Carl Erik Olsen, Johan Springborg, Høgni Weihe, Margareta Zehnder, George W. Francis |
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| Rok vydání: | 1994 |
| Předmět: |
endocrine system
Growth-hormone-releasing hormone receptor Chemistry General Chemical Engineering Molecular Sequence Data luteinizing hormone/choriogonadotropin receptor Gonadotropic cell Peptide Mapping Chromatography Affinity Molecular Weight Follicle-stimulating hormone Biochemistry Thyrotropin-releasing hormone receptor Hormone receptor Pituitary Gland Animals Cattle Electrophoresis Polyacrylamide Gel Amino Acid Sequence Receptor Receptors LHRH hormones hormone substitutes and hormone antagonists Gonadotropin-releasing hormone receptor |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0904-213X |
| Popis: | Luteinizing hormone releasing hormone (LHRH) regulates the release of luteinizing hormone and follicle stimulating hormone from the pituitary. This process takes place through interaction with high affinity membrane receptors. In addition LHRH inhibits the growth of several cancer cell lines through the interaction with M(r) 60,000 LHRH receptors. Here we describe the purification to homogeneity of the M(r) 60,000 bovine pituitary LHRH binding protein in amounts allowing N-terminal sequencing and peptide mapping. The procedure describes solubilization of luteinizing hormone releasing hormone receptors from homogenized bovine pituitaries in an active form by using the detergent Triton X-114. The receptors were retained in the Triton X-114 phase during temperature-dependent phase separation. Preparative phase separations were performed directly on solubilized bovine pituitary extracts. SDS-PAGE of the purified LHRH receptor after LHRH-immobilized affinity chromatography showed the presence of a single band with M(r) 60,000. Partial sequencing of this band after trypsin digestion of gel pieces revealed unknown sequences with a possible homology to other receptors including some G-protein coupled receptors. |
| Databáze: | OpenAIRE |
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