Effects of frutalin and doxorubicin on growth, ultrastructure and gene expression in goat secondary follicles cultured in vitro
| Autor: | E.V. Cunha, A. W. B. Silva, J. R. V. Silva, André Luis Coelho da Silva, L.R.F.M. Paulino, B.R. Silva, J.J.N. Costa, R. van den Hurk, M.A.A. Soares-Costa |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Messenger RNA Dose-Response Relationship Drug General Veterinary Chemistry Galectins Goats Antineoplastic Agents Molecular biology In vitro Tissue Culture Techniques 03 medical and health sciences 030104 developmental biology Gene Expression Regulation Ovarian Follicle Doxorubicin Gene expression Ultrastructure medicine Animals Female RNA Messenger medicine.drug |
| Zdroj: | Research in Veterinary Science. 120:33-40 |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/j.rvsc.2018.08.006 |
| Popis: | This study evaluated the effects of frutalin (0.6, 6.0 or 60.0 μg/mL) and doxorubicin (0.3 μg/mL) on survival, growth and ultrastructure of in-vitro cultured goat secondary follicles. The effects of these substances on the levels of mRNA for Casp3, Casp6, Bax, and Bcl2 were also investigated. Results showed that, after 6 days of culture, frutalin or doxorubicin reduced the percentage of normal follicles (P 0.05), but doxorubicin had higher toxicity than frutalin. Except for follicles cultured with 0.6 μg/mL frutalin, follicular growth rate was reduced after culture with doxorubicin or frutalin (P 0.05). The presence doxorubicin or 60.0 μg/mL frutalin increased the levels of mRNA for Casp3, Casp6, Bax, and Bcl2 (P 0.05). Higher mRNA levels for Casp3, Casp6 and Bax were found in follicles cultured with doxorubicin, but higher levels of Bcl2 mRNA were found in follicles cultured with frutalin (P 0.05). In conclusion, frutalin has lower toxic effects than doxorubicin on secondary follicles cultured in vitro. |
| Databáze: | OpenAIRE |
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