Validation of Quantitative and Qualitative Methods for Detecting Allergenic Ingredients in Processed Foods in Japan
| Autor: | Reiko Teshima, Reiko Adachi, Shinobu Sakai, Hiroshi Akiyama |
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| Rok vydání: | 2012 |
| Předmět: |
Validation study
Screening test Enzyme-Linked Immunosorbent Assay Guidelines as Topic Biology Polymerase Chain Reaction Arthropod Proteins Ingredient Japan Food Labeling Limit of Detection Food allergy Crustacea Food Preserved medicine Animals Humans Food science Food allergens Shellfish business.industry Reproducibility of Results DNA General Chemistry Allergens Legislation Food Food Inspection medicine.disease Biotechnology DNA metabolism Crustacean Proteins Food processing Fast Foods Dietary Proteins General Agricultural and Biological Sciences business Food Hypersensitivity |
| Zdroj: | Journal of Agricultural and Food Chemistry. 61:5675-5680 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf3033396 |
| Popis: | A labeling system for food allergenic ingredients was established in Japan in April 2002. To monitor the labeling, the Japanese government announced official methods for detecting allergens in processed foods in November 2002. The official methods consist of quantitative screening tests using enzyme-linked immunosorbent assays (ELISAs) and qualitative confirmation tests using Western blotting or polymerase chain reactions (PCR). In addition, the Japanese government designated 10 μg protein/g food (the corresponding allergenic ingredient soluble protein weight/food weight), determined by ELISA, as the labeling threshold. To standardize the official methods, the criteria for the validation protocol were described in the official guidelines. This paper, which was presented at the Advances in Food Allergen Detection Symposium, ACS National Meeting and Expo, San Diego, CA, Spring 2012, describes the validation protocol outlined in the official Japanese guidelines, the results of interlaboratory studies for the quantitative detection method (ELISA for crustacean proteins) and the qualitative detection method (PCR for shrimp and crab DNAs), and the reliability of the detection methods. |
| Databáze: | OpenAIRE |
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