YgbQ, a cell division protein in Escherichia coli and Vibrio cholerae , localizes in codependent fashion with FtsL to the division site
| Autor: | Nienke Buddelmeijer, Nicholas Judson, Dana Boyd, John J. Mekalanos, Jonathan Beckwith |
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| Rok vydání: | 2002 |
| Předmět: |
Cell division
Operon Recombinant Fusion Proteins Blotting Western Green Fluorescent Proteins Molecular Sequence Data Cell Cycle Proteins Muramoylpentapeptide Carboxypeptidase Biology medicine.disease_cause Open Reading Frames Bacterial Proteins Multienzyme Complexes Escherichia coli medicine Penicillin-Binding Proteins Amino Acid Sequence Cell Cycle Protein Vibrio cholerae Gene Multidisciplinary Sequence Homology Amino Acid Escherichia coli Proteins Cell Membrane Membrane Proteins DNA Biological Sciences Arabinose Cell biology Luminescent Proteins Phenotype Databases as Topic Hexosyltransferases Microscopy Fluorescence Biochemistry Membrane protein Essential gene Peptidyl Transferases Peptidoglycan Glycosyltransferase Carrier Proteins Cell Division Protein Binding |
| Zdroj: | Proceedings of the National Academy of Sciences. 99:6316-6321 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.092128499 |
| Popis: | YgbQ is a cell division protein in Escherichia coli and Vibrio cholerae . In E. coli the ygbQ gene was discovered as a result of a computer search of the E. coli genome designed to find potential interacting partners for cell division protein FtsL. In V. cholerae , ygbQ was identified as an essential gene by using a transposon that fuses genes to an arabinose promoter. The role of YgbQ in cell division is supported by the following. Cells depleted of YgbQ in both organisms form long filaments, but DNA segregation is not affected. YgbQ localizes to the constriction site in wild-type E. coli cells. Localization of E. coli YgbQ to the constriction site depends on cell division proteins FtsQ and FtsL but not FtsW and FtsI, placing YgbQ in the sequential dependency order of proteins localizing to the division site. Localization of green fluorescent protein-FtsL also depends on YgbQ, indicating that FtsL and YgbQ colocalize to the division site in E. coli . Our results show colocalization of proteins to the bacterial midcell in E. coli and raise the possibility that these proteins interact in a coiled-coil structure. |
| Databáze: | OpenAIRE |
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