Reverse Transcription–Polymerase Chain Reaction Testing on Filter Paper–Dried Serum for Laboratory-Based Dengue Surveillance—American Samoa, 2018
| Autor: | Tyler M. Sharp, Catherine A. Montalbo, Emily J Curren, Brad J. Biggerstaff, William Thane Hancock, Aifili John Tufa, Marc Fischer, June S. Vaifanua-Leo, Carolyn V. Gould, Susan L. Hills |
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| Rok vydání: | 2020 |
| Předmět: |
Serum
Suspected dengue Reference laboratory Dengue virus medicine.disease_cause Sensitivity and Specificity Dengue fever Dengue Virology medicine Humans Serologic Tests Filter paper Reverse Transcriptase Polymerase Chain Reaction business.industry Outbreak Articles medicine.disease American Samoa Reverse transcription polymerase chain reaction Infectious Diseases American samoa Population Surveillance Parasitology business |
| Zdroj: | Am J Trop Med Hyg |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.19-0800 |
| Popis: | Laboratory-based surveillance for arboviral diseases is challenging in resource-limited settings. We evaluated the use of filter paper–dried sera for detection of dengue virus (DENV) RNA during an outbreak in American Samoa. Matched liquid and filter paper–dried sera were collected from patients with suspected dengue and shipped to a reference laboratory for diagnostic testing. RNA was extracted from each sample and tested for DENV RNA by real-time reverse transcription-polymerase chain reaction (RT-PCR). Of 18 RT-PCR–positive liquid specimens, 14 matched filter paper–dried specimens were positive for a sensitivity of 78% (95% CI, 55–91%). Of 82 RT-PCR–negative liquid specimens, all filter paper–dried specimens were negative for a specificity of 100% (95% CI, 96–100%). Shipping of filter paper–dried specimens was similarly timely but less expensive than shipping liquid sera. Using filter paper–dried serum or blood can be a cost-effective and sustainable approach to surveillance of dengue and other arboviral diseases in resource-limited settings. |
| Databáze: | OpenAIRE |
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