The reverse gyrase TopR1 is responsible for the homeostatic control of DNA supercoiling in the hyperthermophilic archaeon Sulfolobus solfataricus
| Autor: | Florence Garnier, Danièle Gadelle, Marc Nadal, Mohea Couturier, Patrick Forterre |
|---|---|
| Přispěvatelé: | Toegepaste Biologische Wetenschappen, Microbiologie |
| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial Hot Temperature Archaeal Proteins ved/biology.organism_classification_rank.species Microbiology DNA gyrase 03 medical and health sciences chemistry.chemical_compound Transcription (biology) Homeostasis Molecular Biology 030304 developmental biology 0303 health sciences biology 030306 microbiology ved/biology DNA Superhelical Topoisomerase Sulfolobus solfataricus DNA replication Archaea Hyperthermophile Cell biology DNA Topoisomerases Type II chemistry biology.protein DNA supercoil DNA DNA Topoisomerases |
| Zdroj: | Molecular microbiologyREFERENCES. 113(2) |
| ISSN: | 1365-2958 |
| Popis: | Maintaining an appropriate DNA topology with DNA-based processes (DNA replication, transcription and recombination) is crucial for all three domains of life. In bacteria, the homeostatic regulation for controlling DNA supercoiling relies on antagonistic activities of two DNA topoisomerases, TopoI and gyrase. In hyperthermophilic crenarchaea, the presence of such a regulatory system is suggested as two DNA topoisomerases, TopoVI and reverse gyrase, catalyze antagonistic activities. To test this hypothesis, we estimated and compared the number of the TopoVI with that of the two reverse gyrases, TopR1 and TopR2, in Sulfolobus solfataricus cells maintained either at 80 or at 88°C, or reciprocally shifted from one temperature to the other. From the three DNA topoisomerases, TopR1 is the only one exhibiting significant quantitative variations in response to the up- and down-shifts. In addition, the corresponding intrinsic activities of these three DNA topoisomerases were tested in vitro at both temperatures. Although temperature modulates the three DNA topoisomerases activities, TopR1 is the sole topoisomerase able to function at high temperature. Altogether, results presented in this study demonstrate, for the first time, that the DNA topological state of a crenarchaeon is regulated via a homeostatic control, which is mainly mediated by the fine-tuning of TopR1. |
| Databáze: | OpenAIRE |
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