A novel enzyme immunoassay for serodiagnosis of Helicobacter pylori infection
| Autor: | Tsuyoshi Yabana, Akira Yachi, Toshiro Sugiyama, Kohzoh Imai, Kenji Yokota, Yoshikazu Takayama, Keiji Oguma, Hirokiyo Yoshida |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Peptic Ulcer
medicine.drug_class Blotting Western Chronic gastritis Enzyme-Linked Immunosorbent Assay Monoclonal antibody Helicobacter Infections Mice Antigen medicine Animals Humans Mice Inbred BALB C Helicobacter pylori Hepatology biology medicine.diagnostic_test Gastroenterology Antibodies Monoclonal biology.organism_classification medicine.disease Antibodies Bacterial Virology Gastritis Immunoassay Chronic Disease Immunology Monoclonal biology.protein Female Antibody medicine.symptom |
| Zdroj: | Gastroenterology. 101:77-83 |
| ISSN: | 0016-5085 |
| Popis: | Helicobacter pylori has recently been implicated as an etiologic agent of gastroduodenal disorders. Comparing the antibody to H. pylori in the sera of patients with that of normal controls by Western blot analysis, a unique antibody was detected in the sera of patients, which reacted with the 25-kilodalton antigen of H. pylori. On the other hand, monoclonal antibody CP3 prepared in the authors' laboratory also recognized the 25-kilodalton antigen of H. pylori. Whether the serum antibody of the patient recognized the CP3 antigen purified by monoclonal antibody CP3 was then examined. Western blot analysis showed that the patient's serum reacted strongly with the affinity-purified CP3 antigen. Using monoclonal antibody CP3, an enzyme-linked immunosorbent assay to detect CP3 antibody in sera was established. In patients with chronic gastritis and gastric ulcers, the titer of CP3 antibody was significantly higher than in normal controls and correlated with the histological grade of antral gastritis. The detection of CP3 antibody in sera is useful in the diagnosis of chronic gastritis and gastric ulcer associated with H. pylori infection and also in evaluation of the grade of gastritis. |
| Databáze: | OpenAIRE |
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