Combined detection of estrogen and tumor markers is an important reference factor in the diagnosis and prognosis of lung cancer

Autor: Yuquan Bai, Jinping Zhao, Li Zhang, Yanhong Wei, Hexiao Tang, Minglin Zhu, Wulin Shen
Rok vydání: 2018
Předmět:
0301 basic medicine
MAPK/ERK pathway
Oncology
Lung Neoplasms
Biochemistry
0302 clinical medicine
Carcinoembryonic antigen
Carcinoma
Non-Small-Cell Lung
biology
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
Middle Aged
Prognosis
Survival Rate
030220 oncology & carcinogenesis
Carcinoma
Squamous Cell
Female
Menopause
Mitogen-Activated Protein Kinases
Signal Transduction
medicine.medical_specialty
medicine.drug_class
Blotting
Western
Enolase
Enzyme-Linked Immunosorbent Assay
GPI-Linked Proteins
03 medical and health sciences
Western blot
Antigen
Antigens
Neoplasm
Internal medicine
Biomarkers
Tumor
medicine
Humans
In patient
Lung cancer
Molecular Biology
Keratin-19
business.industry
Estrogens
Cell Biology
medicine.disease
Carcinoembryonic Antigen
030104 developmental biology
A549 Cells
Estrogen
Phosphopyruvate Hydratase
biology.protein
business
Proto-Oncogene Proteins c-akt
Zdroj: Journal of Cellular Biochemistry. 120:105-114
ISSN: 1097-4644
0730-2312
DOI: 10.1002/jcb.27130
Popis: The correlation between lung cancer tumor markers and sex differences in lung cancer remains a clinical problem that is worthy of further study. This study investigated the significance of the combined detection of 17β-estrogen (E2) and tumor markers in the diagnosis and prognosis of lung cancer. A total of 174 patients, including 117 patients with non-small-cell lung cancer (NSCLC) and 57 patients with benign pulmonary lesions (BPL), were enrolled. An enzyme-linked immunosorbent assay was used to detect the expression of E2, carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), and cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) in patients with NSCLC and BPL to analyze the correlation between E2 and CEA, NSE or CYFRA21-1 expression, and its correlation with clinicopathological features and prognosis. The expression of tumor markers was then examined in different lung cancer cells (A549, H1795, H460, and SK-MES-1). The expression of tumor markers was detected by a real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. The expressions of p-p44/42 mitogen-activated protein kinase (MAPK) and phospho-AKT (p-AKT) were detected by Western blot analysis. The expression levels of E2, CEA, NSE, and CYFRA21-1 in patients with NSCLC were significantly higher than those in patients with BPL ( P < .05); E2 was positively correlated with tumor markers ( P < .01). Patients with a high expression of E2 and tumor markers showed a poor prognosis ( P < .05). RT-quantitative PCR and Western blot analysis showed that the expression levels of CEA, NSE, CYFRA21-1, p-p44/42 MAPK, and p-AKT in the E2 group were higher than those in the other groups ( P < .05). These studies indicate that the interaction of E2 and tumor markers can significantly improve the role of tumor markers in the diagnosis and prognosis of lung cancer.
Databáze: OpenAIRE
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