Kpi, a Chaperone-Usher Pili System Associated with the Worldwide-Disseminated High-Risk Clone Klebsiella Pneumoniae ST-15
| Autor: | Jesús Oteo Iglesias, Jose Ramos Vivas, Pedro J Sola Campoy, María Pérez-Vázquez, Juan A. Vallejo, Alejandro Beceiro, Germán Bou, Laura Álvarez-Fraga, Margarita Poza, Astrid Pérez, Juan C. Vázquez-Ucha, Soraya Rumbo-Feal, Eva Gato, Marta Martínez-Guitián, Bruno Kotska Rodiño-Janeiro, Antonio A. Romero |
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| Přispěvatelé: | Sociedad Espanola de Enfermedades Infecciosas y Microbiologia Clinica, Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, Red Española de Investigación en Patología Infecciosa, European Regional Development Fund, Fundación SEIMC-GESIDA, Gato, Eva [0000-0002-1662-514X], Vázquez-Ucha, Juan C.[0000-0003-4949-0779], Rumbo-Feal, Soraya [0000-0002-1796-1815], Álvarez-Fraga, Laura [0000-0003-3920-5866], Vallejo, Juan Andrés [0000-0002-7581-8654], Martínez-Guitián, Marta [0000-0002-3457-0613], Beceiro, Alejandro [0000-0002-6340-7815], Ramos-Vivas, José [0000-0001-8795-519X], Sola-Campoy, Pedro J.[0000-0002-5881-7377], Pérez-Vázquez,María [0000-0003-0745-8914], Oteo, Jesús [0000-0003-3327-8263], Rodiño-Janeiro, Bruno Kotska [0000-0002-0633-6774], Romero, Antonio [0000-0002-6990-6973], Poza, Margarita [0000-0001-9423-7268], Bou, Germán [0000-0001-8837-0062], Perez, Astrid [0000-0003-1809-3332], Gato, Eva, Vázquez-Ucha, Juan C., Rumbo-Feal, Soraya, Álvarez-Fraga, Laura, Vallejo, Juan Andrés, Martínez-Guitián, Marta, Beceiro, Alejandro, Ramos-Vivas, José, Sola-Campoy, Pedro J., Pérez-Vázquez,María, Oteo, Jesús, Rodiño-Janeiro, Bruno Kotska, Romero, Antonio, Poza, Margarita, Bou, Germán, Perez, Astrid, Instituto de Salud Carlos III - ISCIII, European Regional Development Fund (ERDF/FEDER) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Operon
Klebsiella pneumoniae Chaperone-usher pili system Population Pathogenesis Bacterial Adhesion Pilus Cell Line Microbiology Mice 03 medical and health sciences Antibiotic resistance Drug Resistance Multiple Bacterial Animals Humans education Pathogen Phylogeny 030304 developmental biology Mice Inbred BALB C 0303 health sciences education.field_of_study Multidisciplinary biology 9. Industry and infrastructure 030306 microbiology Biofilm Epithelial Cells ST-15 high-risk clone biology.organism_classification Phenotype Anti-Bacterial Agents Klebsiella Infections 3. Good health Europe Disease Models Animal Carbapenems A549 Cells Genes Bacterial Biofilms Fimbriae Bacterial GI tract colonization Female Gene Deletion Molecular Chaperones Multilocus Sequence Typing |
| Zdroj: | RUC: Repositorio da Universidade da Coruña Universidade da Coruña (UDC) Repisalud Instituto de Salud Carlos III (ISCIII) Digital.CSIC. Repositorio Institucional del CSIC instname RUC. Repositorio da Universidade da Coruña |
| ISSN: | 2016-2952 |
| Popis: | Control of infections caused by carbapenem-resistant Klebsiella pneumoniae continues to be challenging. The success of this pathogen is favored by its ability to acquire antimicrobial resistance and to spread and persist in both the environment and in humans.The emergence of clinically important clones, such as sequence types 11, 15, 101, and 258, has been reported worldwide. However,the mechanisms promoting the dissemination of such highrisk clones are unknown. Unraveling the factors that play a role in the pathobiology and epidemicity of K. pneumoniae is therefore important for managing infections. To address this issue, we studied a carbapenem-resistant ST-15 K. pneumoniae isolate (Kp3380) that displayed a remarkable adherent phenotype with abundant pilus-like structures. Genome sequencing enabled us to identify a chaperone-usher pili system (Kpi) in Kp3380. Analysis of a large K. pneumoniae population from 32 European countries showed that the Kpi system is associated with the ST-15 clone. Phylogenetic analysis of the operon revealed that Kpi belongs to the littlecharacterized γ2-fimbrial clade. We demonstrate that Kpi contributes positively to the ability of K.pneumoniae to form biofilms and adhere to different host tissues. Moreover, the in vivo intestinal colonizing capacity of the Kpi-defective mutant was significantly reduced, as was its ability to infect Galleria mellonella. The findings provide information about the pathobiology and epidemicity of Kpi+ K. pneumoniae and indicate that the presence of Kpi may explain the success of the ST-15 clone. Disrupting bacterial adherence to the intestinal surface could potentially target gastrointestinal colonization. This research was supported by Projects p-01216A and IJCI-2016-29524 (to A.P.), funded by the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) and the Minestry of Economy and Competetiveness(MINECO), respectively. It was also supported by Projects PI11/01034 (to M.P.), PI14/00059 and PI17/1482 (to M.P. and A.B.), and PI18/00501 (to G.B.),included in the National Plan for Scientific Research, Development and Technological Innovation 2013-2016 and funded by the Instituto de Salud Carlos III (ISCIII) and Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía, Industria y Competitividad, Spanish Network for Research in Infectious Diseases (REIPI RD16/0016/006) cofinanced by European Development Regional Fund “A way to achieve Europe” and operative program Intelligent Growth 2014-2020. Grant BFU2016-77835-R of the MINECO (to A.R.) also supported this research. E.G. was financially supported by the SEIMC project. J.C.V.-U. was financially supported by the PFIS (Contratos Predoctorales de Formación en Investigación en Salud) program (F18/00315);J.A.V. was financially supported by IN607A 2016/22; M.M.-G. was financially supported by a Clara Roy grant (SEIMC); A.B. was financially supported by the Miguel Servet program (ISCIII, Spain); B.K.R.-J. was financially supported by Marie S. Curie Action SaPhaDe project (MSCA-IF-GF-836754); and A.P. was financially supported by the Juan de la Cierva program (MINECO, IJCI-2016-29524). |
| Databáze: | OpenAIRE |
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