Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation
| Autor: | Lucas Carneiro Costa, José Mauro Granjeiro, Letícia Ladeira Bonato, Julie Calixto Lobo, Valquiria Quinelato, Marcos Alexandre da Fonseca, Aldir Nascimento Machado, Priscila Ladeira Casado, Diego Pinheiro Aguiar, Priscilla Gonçalves Lomardo, Aristides da Rosa Pinheiro, Fernando Luiz Duarte de Almeida, Miriam Jordão, Thays Cristine dos Santos Vieira, Telma Aguiar |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases Male medicine.medical_specialty Blotting Western Inflammation Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine Osteoprotegerin Internal medicine medicine Mucositis Humans RNA Messenger General Dentistry Aged Periodontitis Dental Implants RANK ligand biology business.industry RANK Ligand Mouth Mucosa osseointegration chronic periodontitis 030206 dentistry Middle Aged medicine.disease Chronic periodontitis Immunohistochemistry 030104 developmental biology Endocrinology Real-time polymerase chain reaction mucositis RANKL osteoprotegerin Chronic Periodontitis biology.protein Female medicine.symptom business |
| Zdroj: | Brazilian Dental Journal, Volume: 29, Issue: 1, Pages: 14-22, Published: FEB 2018 Brazilian Dental Journal v.29 n.1 2018 Brazilian Dental Journal Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
| ISSN: | 1806-4760 |
| Popis: | tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p |
| Databáze: | OpenAIRE |
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