An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii
| Autor: | Jacqueline Fletcher, William Dolezal, Astri Wayadande, Ulrich Melcher, María de la Paz Giménez Pecci, Graciela Laguna, Pablo Carpane |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Costa Rica
DNA Bacterial KUNKELII Genotype Spiroplasma Argentina Plant Science Variación Genética Genome Zea mays Spiroplasma kunkelii Bacterial Proteins MOLLICUTE Genetic variation Enfermedades de las Plantas Polymorphism Maíz Gene Mexico Phylogeny Polimorfismo Disease Resistance Plant Diseases Genetics Host resistance Insect cell Polymorphism Genetic biology Geography GENOMIC Genetic Variation Sequence Analysis DNA biology.organism_classification United States Maize Otras Agricultura Silvicultura y Pesca Plant Leaves SPIROPLASMA CIENCIAS AGRÍCOLAS purl.org/becyt/ford/4.1 [https] Agricultura Silvicultura y Pesca Agronomy and Crop Science purl.org/becyt/ford/4 [https] Brazil Genome Bacterial |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Phytopathology 103 (2) : 129-134 (February 2013) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA |
| ISSN: | 0031-949X |
| Popis: | Corn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components that could affect the stability of host resistance, we assessed the genome variability of the etiologic agent, Spiroplasma kunkelii. Isolates were obtained from a number of areas, and characterized molecularly by amplification of several regions of the spiroplasma chromosome and sequencing of specific gene fragments. The degree of polymorphism between isolates of different geographic origins was low, and the level of genomic variability was similar within isolates of different countries. Polymorphism among isolates was found in viral insertions and in the sequence of Skarp, a gene that encodes a membrane protein implicated in attachment to insect cells. The results suggest that the genome composition of this species is highly conserved among isolates. Hence, it is unlikely that the instability of maize resistance is due to generation of new pathotypes of S. kunkelii. Instead, other components of this complex pathosystem could account for the breakdown of resistance. Fil: Carpane, Pablo. Oklahoma State University; Estados Unidos Fil: Melcher, Ulrich. Oklahoma State University; Estados Unidos Fil: Wayadande, Astri. Oklahoma State University; Estados Unidos Fil: Giménez, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronomicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronomicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dolezal, William. Pioneer Hi-Bred International; Estados Unidos Fil: Fletcher, Jacqueline. Oklahoma State University; Estados Unidos |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|