Influence of bioactive glass-coating of zirconia implant surfaces on human osteoblast behavior in vitro
| Autor: | Horst Fischer, Marc Weber, Jens Fischer, Petra Müller, Fredy Schmidli, Nadja Rohr, J. Barbara Nebe |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Surface Properties 02 engineering and technology engineering.material Osseointegration law.invention 03 medical and health sciences 0302 clinical medicine Coating law Surface roughness medicine Humans General Materials Science Cubic zirconia Viability assay General Dentistry Cell Proliferation Titanium Osteoblasts Biomaterial Osteoblast 030206 dentistry 021001 nanoscience & nanotechnology medicine.anatomical_structure Chemical engineering Mechanics of Materials Bioactive glass engineering Microscopy Electron Scanning Zirconium 0210 nano-technology |
| Zdroj: | Dental materials : official publication of the Academy of Dental Materials. 35(6) |
| ISSN: | 1879-0097 |
| Popis: | Objective The recently developed bioactive glass PC-XG3, which is suitable to coat zirconia implant surfaces with high adhesion strength may reduce the time of osseointegration and the marginal bone loss following implantation. The glass composition has been previously evaluated for cytotoxicity on fibroblast cells, and will now be used to evaluate the cell behavior of osteoblast cells. Methods Three different surface morphologies were created with PC-XG3 on zirconia discs. A clinically tested zirconia implant surface as well as polished and machined zirconia served as a reference. Cell viability after 24 h, cell spreading after 30 min and 24 h and the respective morphology of human osteoblasts using scanning electron microscopy were evaluated. Additionally, the corrosive process of PC-XG3 in cell culture medium up to 7 d was measured. Results Initial cell behavior of human osteoblasts was not accelerated by the PC-XG3 surface when compared to zirconia. Additionally, it was found that a decreased surface roughness promoted initial cell spreading. Storage in cell culture medium resulted in the accumulation of C and N on the bioglass surface while Mg, Si, K and Ca were decreased and crack formation was observed. Significance Since initial spreading quality to a biomaterial is a crucial factor that will determine the subsequent cell function, proliferation, differentiation, and viability it can be assumed that a coating of zirconia implants with this bioactive glass will unlikely reduce osseointegration time. |
| Databáze: | OpenAIRE |
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