Bilophila wadsworthiaClinical Isolates Compared by Polymerase Chain Reaction Fingerprinting
| Autor: | Ellie J. C. Goldstein, M. C. Claros, S. Hunt Gerardo, Marie K. Hudspeth, M. Marina, Diane M. Citron |
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| Rok vydání: | 1997 |
| Předmět: |
Microbiology (medical)
clone (Java method) Gram-Negative Anaerobic Bacteria Strain (chemistry) Brain Abscess Biology DNA Fingerprinting Polymerase Chain Reaction law.invention Microbiology Bilophila wadsworthia Infectious Diseases DNA profiling law Humans Primer (molecular biology) Cholesteatoma Polymerase chain reaction |
| Zdroj: | Clinical Infectious Diseases. 25:S291-S294 |
| ISSN: | 1537-6591 1058-4838 |
| DOI: | 10.1086/516229 |
| Popis: | Bilophila wadsworthia isolates recovered from a right-ear cholesteatoma and brain abscess of the same patient were analyzed by means of polymerase chain reaction (PCR) fingerprinting with single primers (T3B and M13 core) to ascertain if they originated from the same clone. Their PCR fingerprint profiles were compared with those of three additional B. wadsworthia clinical isolates and the type strain (ATCC 49260). The two isolates from the same patient produced PCR fingerprint profiles identical to each other, regardless of which primer was used. All isolates' PCR fingerprint profiles, with use of either the T3B or M13 core primer, shared some major and minor bands. However, differences in additional major and minor bands distinguished each of the additional isolates, suggesting that there are different subgroups of B. wadsworthia. |
| Databáze: | OpenAIRE |
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