Extra Domain B Fibronectin as a Target for Near-Infrared Fluorescence Imaging of Rheumatoid Arthritis Affected Joints In Vivo
| Autor: | Ole Gemeinhardt, Michael Schirner, Jan Voigt, Kai Licha, Ines Gemeinhardt, Vollmer Sonja, Axel Vater, Jörg Schnorr, Rainer Macdonald, Matthias Taupitz, Bernd Ebert |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Near-Infrared Fluorescence Imaging
Pathology medicine.medical_specialty lcsh:Medical technology Biomedical Engineering Arthritis Tarsus Animal Mice In vivo Synovitis medicine Animals Whole Body Imaging Radiology Nuclear Medicine and imaging Immunoglobulin Fragments lcsh:QH301-705.5 Fluorescent Dyes Spectroscopy Near-Infrared biology Chemistry Cartilage Synovial Membrane Ear Carbocyanines Condensed Matter Physics medicine.disease Arthritis Experimental Immunohistochemistry Fibronectins Rats Fibronectin medicine.anatomical_structure lcsh:Biology (General) lcsh:R855-855.5 Rats Inbred Lew Rheumatoid arthritis biology.protein Molecular Medicine Female Sulfonic Acids Molecular imaging Biomarkers Biotechnology |
| Zdroj: | Molecular Imaging, Vol 8 (2009) |
| ISSN: | 1536-0121 |
| Popis: | We investigated a molecular imaging approach for the detection of collagen-induced arthritis in rats by targeting the extra domain B (ED-B) of the extracellular matrix protein fibronectin. ED-B is a highly conserved domain (identical in human and rats) that is produced by alternative splicing during embryonic development and during vascular remodeling such as angiogenesis. The hallmark of rheumatoid arthritis is synovitis leading to both angiogenesis in the synovium and the promotion of cartilage and bone disruption. For in vivo diagnostics, the ED-B-binding single-chain antibody fragment AP39 was used as a targeting probe. It was covalently linked to the near-infrared dye tetrasulfocyanine (TSC) to be visualized by near-infrared fluorescence imaging. The resulting AP39-TSC conjugate was intravenously administered to rats with collagen-induced arthritis and the respective controls. Ovalbumin-TSC was used as control conjugate. Optical imaging over a time period of 24 hours using a planar imaging setup resulted in a clear enhancement of fluorescence intensity in joints with moderate to severe arthritis compared with control joints between 3 and 8 hours postinjection. Given that AP39 is a fully human antibody fragment, this molecular imaging approach for arthritis detection might be translated to humans. |
| Databáze: | OpenAIRE |
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