First-time isolation of Candida dubliniensis from plaque and carious dentine of primary teeth
| Autor: | S. Nietzsche, Susanne Kneist, A. Borutta, Angelika Callaway, H. Küpper, M. Kostrzewa, B. W. Sigusch |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Saliva Colony Count Microbial Dental Plaque Dentistry Germ tube Dental Caries Microbiology Streptococcus mutans stomatognathic system Medicine Humans Dentistry (miscellaneous) Tooth Deciduous Candida albicans Candida Streptococcus sobrinus biology Dentition business.industry DMF Index biology.organism_classification Isolation (microbiology) medicine.disease Corpus albicans Bacterial Load stomatognathic diseases Lactobacillus Lacticaseibacillus casei Child Preschool Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Pediatrics Perinatology and Child Health Dentin Microscopy Electron Scanning business Candida dubliniensis Early childhood caries |
| Zdroj: | European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry. 16(4) |
| ISSN: | 1818-6300 |
| Popis: | To determine those organisms of the genus Candida associated with dental caries by investigating samples from active carious lesions. Within the genus Candida, the species Candida albicans and Candida dubliniensis are capable of forming chlamydospores and germ tubes. Until it became possible in 1995 to differentiate between the two species taxonomically, C. dubliniensis was falsely identified as C. albicans. Whilst the importance of C. albicans for rapidly progressing early childhood caries (ECC) has been recognised, so far there have been only reports about C. dubliniensis in connection with children/mothers who have been infected with HIV or already developed AIDS. In the present study, C. dubliniensis was for the first time isolated from plaque and carious dentine of a healthy five-year-old boy. As part of the investigation, a number of samples were collected from individual children affected by active dental caries. Amongst the samples, one in particular indicated that Candida species might be involved. The patient was a five-year-old boy with ECC of the primary dentition, scheduled for restorative treatment under general anaesthesia. Before treatment, a salivary, plaque (region of 54/55) and soft carious dentine sample from the tooth 51 was taken before extraction. The counts of yeasts, lactobacilli (LB) and mutans streptococci were determined in the samples. The boy’s dmft was 11, which was dominated by the d component. In the saliva of the boy, LB and mutans streptococci (MS) were detected. In plaque and carious dentine, MS and most interestingly C. dubliniensis were present. The yeasts were visualised in carious dentine by means of scanning electron micrographs. Plaque and carious dentine may be a further habitat of C. dubliniensis. |
| Databáze: | OpenAIRE |
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