Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord

Autor: Cristian Marcelo Sobarzo, Livia Lustig, Diego Moreno, Vanesa Anabella Guazzone, Marcelo Rodríguez Peña
Rok vydání: 2019
Předmět:
Male
Pathology
030232 urology & nephrology
Cell Count
Orchitis
mast cells
lcsh:RC870-923
Severity of Illness Index
male infertility
0302 clinical medicine
Fibrosis
KETOTIFEN FUMARATE
Testicular torsion
Hypersensitivity
Delayed
Spermatic Cord Torsion
Epididymis
Epididymitis
Immunity
Cellular
030219 obstetrics & reproductive medicine
TESTIS
Vaccination
Otras Medicina Básica
purl.org/becyt/ford/3.1 [https]
General Medicine
Medicina Básica
Seminiferous tubule
medicine.anatomical_structure
Histamine H1 Antagonists
purl.org/becyt/ford/3 [https]
Original Article
Ketotifen Fumarate
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
Urology
ketotifen fumarate
testis
Spermatic cord
Autoimmune Diseases
03 medical and health sciences
Atrophy
orchitis
testicular cord torsion
medicine
Animals
Ketotifen
MALE INFERTILITY
TESTICULAR CORD TORSION
MAST CELLS
business.industry
medicine.disease
lcsh:Diseases of the genitourinary system. Urology
Rats
Histopathology
ORCHITIS
business
Zdroj: Asian Journal of Andrology
Asian Journal of Andrology, Vol 22, Iss 1, Pp 112-117 (2020)
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 1745-7262
Popis: The aim of this work was to study effects of ketotifen fumarate (KF) on prevention of tissue damage in testes of rats with experimental autoimmune orchitis (EAO) and on the contralateral testis in a model of prolonged testicular cord torsion (TCT). Rats with EAO or TCT were injected intraperitoneally once daily with KF or saline solution (vehicle group). Incidence and severity of testicular damage were evaluated by histopathology using an EAO score or a Johnsen score. Mast cells (MC) were identified by histochemistry and quantified. In EAO model, KF significantly reduced severity of histopathological testicular damage compared to rats in the vehicle group. KF also reduced the number of testicular MC compared to vehicle group. Similarly, in TCT model, multifocal damage of the contralateral testis was observed 30 days after testicular torsion characterized by sloughing of the germinal epithelium, seminiferous tubule atrophy, and interstitial edema. Focal signs of inflammation and fibrosis of seminiferous tubular walls were also observed. In contrast, sections of contralateral testis of rats injected with KF and killed 30 days after surgery showed normal histological features. A significant decrease in the number of MC was observed in rats treated with KF compared to untreated animals. In conclusion, we demonstrated that treatment with KF reduced testicular inflammatory process and MC infiltrates in both EAO and TCT models. The results suggest a promising treatment for infertile male patients with testicular pathologies associated with inflammation and germ cell loss. Fil: Moreno, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina Fil: Sobarzo, Cristian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina Fil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina Fil: Rodriguez Peña, Marcelo Javier. Ministerio de Defensa. Ejercito Argentino. Hospital Militar Campo de Mayo Cirujano Primero Doctor Juan Madera; Argentina Fil: Guazzone, Vanesa Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Databáze: OpenAIRE
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