Comparison of the Post-transcriptional Regulation of the mRNAs for the Surface Proteins PSA (GP46) and MSP (GP63) ofLeishmania chagasi
| Autor: | Mary E. Wilson, Jeffrey K. Beetham, John E. Donelson, Karen S. Myung |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Molecular Sequence Data
Protozoan Proteins Trypanosoma brucei Transfection Biochemistry Pentapeptide repeat Plasmid Genes Reporter parasitic diseases Animals RNA Messenger RNA Processing Post-Transcriptional 3' Untranslated Regions Molecular Biology Post-transcriptional regulation Leishmania chemistry.chemical_classification Reporter gene Membrane Glycoproteins Base Sequence biology Metalloendopeptidases Cell Biology Leishmania chagasi beta-Galactosidase biology.organism_classification Molecular biology Amino acid chemistry Glycoprotein Gene Deletion Plasmids Protein Binding |
| Zdroj: | Journal of Biological Chemistry. 277:16489-16497 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m200174200 |
| Popis: | MSP (GP63) and PSA (GP46) are abundant 63- and 46-kDa glycolipid-anchored proteins on the surface of the promastigote form of most Leishmania species. MSP is a zinc metalloprotease that confers resistance to host complement-mediated lysis. PSA contains internal repeats of 24 amino acids, and its function is unknown. The steady state levels of mRNAs for both glycoproteins are regulated post-transcriptionally, resulting in about a 30-fold increase as Leishmania chagasi promastigotes grow in vitro from logarithmic phase to stationary phase. Previous studies showed the 3'-untranslated regions (3'-UTRs) of these mRNAs are essential for this post-transcriptional regulation. These two 3'-UTRs of 1.0 and 1.3 kilobases were cloned immediately downstream of a beta-galactosidase reporter gene in a plasmid, and segments were systematically deleted to examine which portions of the 3'-UTRs contribute to the post-transcriptional regulation. The 92-nucleotide segment of greatest similarity between the two 3'-UTRs was deleted without loss of regulation, but the segments flanking this similarity region have positive regulatory elements essential for the regulation. We propose that similar, but non-identical, molecular mechanisms regulate the parallel expression of these two L. chagasi mRNAs despite their lack of sequence identity. These post-transcriptional mechanisms resemble the mechanism recently suggested for the regulation of mRNAs encoding the dipeptide (EP) and pentapeptide (GPEET) repeat proteins in Trypanosoma brucei that involves interactions between positive and negative regulatory elements in the 3'-UTR. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|