Regulatory Role of the C-Terminus of the ε Subunit from the Chloroplast ATP Synthase
| Autor: | Kristine F. Nowak, Richard E. McCarty |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
ATP synthase
biology Chemiosmosis Hydrolysis Proteins Photophosphorylation Thylakoids Biochemistry Peptide Fragments Recombinant Proteins Protein Structure Tertiary Adenosine Triphosphate Spinacia oleracea ATP hydrolysis ATP synthase gamma subunit F-ATPase biology.protein Chloroplast Proton-Translocating ATPases V-ATPase Magnesium Ca(2+) Mg(2+)-ATPase ATP synthase alpha/beta subunits |
| Zdroj: | Biochemistry. 43:3273-3279 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi035820d |
| Popis: | The ATP synthases from chloroplasts and Escherichia coli are regulated by several factors, one of which is the epsilon subunit. This small subunit is also required for ATP synthesis. Thylakoid membranes reconstituted with CF1 lacking the epsilon subunit (CF1-epsilon) exhibit no ATP synthesis and very high ATP hydrolysis. Either native or recombinant epsilon restores ATP synthesis and inhibits ATP hydrolysis. Previously, we showed that truncated epsilon, lacking the last 45 C-terminal amino acids, restored ATP synthesis to membranes reconstituted with CF1-epsilon but was not an efficient inhibitor of ATP hydrolysis. In this paper, we show that this truncated epsilon is unable to inhibit ATP hydrolysis when Mg(2+) is the divalent cation present, both for the enzyme in solution and on the thylakoid membrane. In addition, the rate of reduction of the disulfide bond of the gamma subunit by dithiothreitol is not decreased by truncated epsilon, although full-length epsilon greatly impedes reduction. Thylakoid membranes can synthesize ATP at the expense of proton gradients generated by pH transitions in the dark. Our reconstituted membranes are able to produce a limited amount of ATP under these "acid-bath" conditions, with approximately equal amounts produced by the membranes containing wild-type epsilon and those containing truncated epsilon. However, the membranes containing truncated epsilon exhibit much higher background ATP hydrolysis under the same acid-bath conditions, leading to the conclusion that, without the C-terminus of epsilon, the CF1CFo is unable to check unwanted ATP hydrolysis. |
| Databáze: | OpenAIRE |
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