Homologous and Heterologous Borrelia burgdorferi Challenge of Infection-Derived Immune Rabbits Using Host-Adapted Organisms
| Autor: | Xiao-Yang Wu, Michael A. Lovett, James N. Miller, David R. Blanco, Ellen S. Shang |
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| Rok vydání: | 2001 |
| Předmět: |
Lipoproteins
Blotting Western Immunology Heterologous Spirochaetaceae Polymerase Chain Reaction Microbiology Lyme disease Immune system Immunity medicine Animals Borrelia burgdorferi Lyme Disease biology Lyme Disease Vaccines biology.organism_classification medicine.disease Adaptation Physiological Virology Bacterial vaccine DNA Topoisomerases Type II Infectious Diseases DNA Gyrase Microbial Immunity and Vaccines Antigens Surface Bacterial Vaccines Erythema chronicum migrans Parasitology Rabbits medicine.symptom Bacterial Outer Membrane Proteins |
| Zdroj: | Infection and Immunity. 69:593-598 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.69.1.593-598.2001 |
| Popis: | We have recently found that strain B31 infection-immune rabbits are completely protected against homologous challenge with large numbers (>10 6 ) of host-adapted Borrelia burgdorferi (HAB) (E. S. Shang, C. I. Champion, X. Wu, J. T. Skare, D. B. Blanco, J. N. Miller, and M. A. Lovett, Infect. Immun. 68:4189–4199, 2000). In this study, we have extended these findings to determine whether B31 strain infection-immune rabbits are also protected against heterologous HAB challenge. Infection-immune rabbits challenged with large numbers (>10 6 ) of homologous HAB strain B31 were completely protected from erythema migrans (EM) and skin and disseminated infection. In contrast, infection-immune rabbits challenged with heterologous HAB strains N40 and Sh-2-82 were completely susceptible to EM and skin and disseminated infection; challenge with strain 297 also resulted in EM and infection of the skin and viscera, but clearance of infection occurred 3 weeks postchallenge. These findings confirm that immunity elicited in rabbits by B31 strain infection confers complete protection against large-dose homologous HAB challenge but not against a heterologous strain. |
| Databáze: | OpenAIRE |
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