Induction of both cytosolic phospholipase A2 and prostaglandin H synthase-2 by interleukin-1 beta in WISH cells in inhibited by dexamethasone
| Autor: | Phillip R. Bennett, D M Slater, Leslie Myatt, Song Xue |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Molecular Sequence Data Cycloheximide Biochemistry Dexamethasone Dinoprostone Phospholipases A Cell Line chemistry.chemical_compound Endocrinology Phospholipase A2 Cytosol Internal medicine medicine Protein biosynthesis Humans Cyclooxygenase Inhibitors Amnion RNA Messenger Gene Glucocorticoids Messenger RNA biology Base Sequence Glyceraldehyde-3-Phosphate Dehydrogenases Molecular biology Phospholipases A2 chemistry Prostaglandin-Endoperoxide Synthases Enzyme Induction biology.protein Glucocorticoid medicine.drug Interleukin-1 |
| Zdroj: | Prostaglandins. 51(2) |
| ISSN: | 0090-6980 |
| Popis: | In previous studies we have shown that IL-1 beta induced both PGE2 release and total cellular cPLA2 activity and cPLA2 protein synthesis in human amnion-derived WISH cells. In this study, the effect of IL-1 beta on cPLA2 and PGHS-2 mRNA expression was investigated. Using RT-PCR, we found that IL-1 beta (0.1 ng/ml) coordinately induced both cPLA2 and PGHS-2 mRNA expression within 2 hours. The synthetic glucocorticoid dexamethasone (10(-10)-10(-6)M) inhibited IL-1 beta-induced cPLA2 and PGHS-2 mRNA expression activity and protein synthesis and PGE2 release in a concentration dependent manner. In the absence of IL-1 beta, dexamethasone alone (10(-6)M) inhibited basal cPLA2 activity, mRNA expression and protein synthesis. In addition, cycloheximide (5 micrograms/ml) apparently superinduced, but actinomycin D (2 micrograms/ml) inhibited IL-1 beta-induced cPLA2 and PGHS-2 mRNA expression suggesting that both are immediate early genes and a transcriptional mechanism is involved in the induction of both cPLA2 and PGHS-2 mRNA by IL-1 beta. |
| Databáze: | OpenAIRE |
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