Cloning and structural analysis of the gene encoding the ribosomal protein S6 from the parasite Leishmania infantum
| Autor: | Soraya Taladriz, Alberto Marquet, Vicente Larraga, Gloria Gonzalez-Aseguinolaza |
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| Přispěvatelé: | Instituto de Salud Carlos III, González-Aseguinolaza, Gloria [0000-0002-1600-4562], Larraga, Vicente [0000-0003-1260-7400], González-Aseguinolaza, Gloria, Larraga, Vicente |
| Rok vydání: | 1998 |
| Předmět: |
Ribosomal Proteins
Identification Translation Protein family Donovani Genes Protozoan Molecular Sequence Data Restriction Mapping Biophysics Biology Biochemistry Retinoblastoma-like protein 1 Messenger-RNA Ribosomal protein Sequence HSPA2 SNAP23 Animals Humans Amino Acid Sequence Phosphorylation Elongation Cloning Molecular Leishmania infantum Molecular Biology Alignment HSPA9 Genetics Ribosomal Protein S6 Base Sequence Sequence Homology Amino Acid Promastigotes Cell Biology Autophagy-related protein 13 Recombinant Proteins GPS2 Stimulation Sequence Alignment |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0006-291X |
| Popis: | 5 p.-4 fig. We have cloned the S6 ribosomal protein encoding gene from a Leishmania infantum cDNA library. This parasite protozoon, responsible for leishmaniasis in Europe, is able to undergo developmental changes in vitro and results a good model to study cell differentiation processes. The LiS6 protein sequence indicates its pertinence to the S6 protein family, related to the early mechanisms of cell division, differentiation and activation, and shows an intermediate position between the yeasts and higher eukaryotes. Thus, LiS6 protein has the same amino acid length as that of the higher eukaryotes and certain common features such nucleus entrance sequences and several kinase phosphorylation sites. However, the key functional protein kinase C phosphorylation sites are at different locations and present several threonine instead of the usual serine residues. The gene structural analysis suggest the presence of three different encoding genes that do not present remarkable changes along the different phases of the parasite. This research has been supported by the grant # 97/0492 from the Fondo de Investigación Sanitaria (FIS) of the Ministry of Health. |
| Databáze: | OpenAIRE |
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