Characterization of two endoglucanases for the classification of the earthworm, Eisenia fetida Waki
| Autor: | Shin-ichi Akazawa, Keisuke Yokoyama, Wataru Ogasawara, Tomoya Kobayashi, Jun Yarimizu, Yuki Ikarashi, Yasushi Morikawa, Hikaru Nakazawa |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Eisenia fetida Eisenia andrei Zoology Applied Microbiology and Biotechnology Biochemistry Pichia Substrate Specificity Analytical Chemistry Electron Transport Complex IV 03 medical and health sciences Human health Cellulase Fibrinolytic Agents Escherichia coli Animals Humans Cloning Molecular Oligochaeta Glucans Molecular Biology Phylogeny Enzyme Assays biology business.industry Organic Chemistry Earthworm 04 agricultural and veterinary sciences General Medicine biology.organism_classification Recombinant Proteins Biotechnology Isoenzymes Kinetics 030104 developmental biology Gene Expression Regulation Eisenia 040103 agronomy & agriculture Fibrinolytic enzyme 0401 agriculture forestry and fisheries Xylans business Fibrinolytic agent |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 80:55-66 |
| ISSN: | 1347-6947 0916-8451 |
| Popis: | Eisenia fetida and Eisenia andrei are vermicomposting species that are used as model animals for testing chemical material toxicology. Eisenia spp. are grown commercially in various fields in Japan. However, these two species have not been classified because it is difficult to distinguish them morphologically; thus, all bred earthworms are called E. fetida. However, it has been proposed that these two species have different expression regulation mechanisms. Here, we classified a sample of earthworms purchased from several farms, confirming that both E. fetida and E. andrei are present in Japanese earthworm breeding programs. We also characterized two highly active endoglucanases (EfEG1 and EfEG2) from the E. fetida Waki strain, which contained strong fibrinolytic enzymes for improving human health. We confirmed that EfEG1 is 1371 bp long and belongs to GHF9. Thus, E. fetida Waki may have commercial application for biomass utilization and as a dietary health supplement. |
| Databáze: | OpenAIRE |
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