Investigating selective media for optimal isolation of Brucella spp. in South Africa
| Autor: | Maphuti Betty Ledwaba, Okechukwu C. Ndumnego, Awoke K. Gelaw, Henriette van Heerden, Itumeleng Matle |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Fastidious organism
Veterinary medicine Brucella ovis bacterial isolation Biovar Brucella Brucellosis South Africa 03 medical and health sciences Yeasts medicine Bovine brucellosis Ovis Original Research 030304 developmental biology 0303 health sciences lcsh:Veterinary medicine General Veterinary biology 030306 microbiology selective media Fungi General Medicine Raw milk medicine.disease biology.organism_classification Isolation (microbiology) Anti-Bacterial Agents Culture Media Bacterial isolation lcsh:SF600-1100 bovine brucellosis |
| Zdroj: | Onderstepoort Journal of Veterinary Research, Volume: 87, Issue: 1, Pages: 1-9, Published: 2020 Onderstepoort Journal of Veterinary Research, Vol 87, Iss 1, Pp e1-e9 (2020) The Onderstepoort Journal of Veterinary Research |
| Popis: | Bovine brucellosis in South Africa is caused mainly by Brucella abortus biovar (bv.) 1 and less frequently by B. abortus bv. 2. Bacterial isolation is regarded as the gold standard for diagnosis of Brucella species; however, it is not very sensitive. The aim of this study was to determine the selective medium with optimum antibiotic composition that will allow the growth of Brucella species (spp.) while inhibiting moulds, yeast and most, if not all, Gram-negative contaminants in South Africa. In the controlled experiment, modified Agrifood Research and Technology Center of Aragon (CITA) medium (mCITA) seemed to be the optimum selective medium for isolation of Brucella spp. as compared with Farrell’s medium (FM) and modified Thayer Martin (mTM), while FM inhibited the growth of most fungal and bacterial contaminants. Mean comparison between the three media used to culture B. abortus resulted in lower mean difference ranging from 0 to 2.33. In case of Brucella ovis, high mean difference was obtained when comparing FM with mCITA (10.33) and mTM (12). However, the mean differences of 0.67 and 1.67 were obtained when comparing mCITA and mTM media used to, respectively, culture pasteurised and raw milk spiked with B. ovis. Further optimisation at the Agricultural Research Council – Onderstepoort Veterinary Research Institute resulted in a comparable performance between FM and mCITA; however, mCITA allowed optimal growth of the fastidious B. ovis, which is generally inhibited on FM. Generally, mCITA seemed to be the optimum selective medium for isolation of Brucella spp., while FM inhibits the growth of most fungal and bacterial contaminants. Thus, veterinary laboratories can use mCITA and/or FM but should take into consideration the detection of factious Brucella isolated in the country or region. |
| Databáze: | OpenAIRE |
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