Pigment structure in the light-harvesting protein of the siphonous green alga Codium fragile
| Autor: | Andrew Gall, Ritsuko Fujii, Cristian Ilioaia, Manuel J. Llansola-Portoles, Andrew A. Pascal, Simona Streckaite, Soichiro Seki, Bruno Robert |
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| Přispěvatelé: | Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire Bioénergétique Membranaire et Stress (LBMS), Département Biochimie, Biophysique et Biologie Structurale (B3S), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Chlorophyll b Chlorophyll Lutein Resonance Raman Codium fragile Photosystem II Stereochemistry [SDV]Life Sciences [q-bio] Population Biophysics Light-Harvesting Protein Complexes Siphonous green algae Xanthophylls Photosynthesis 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Pigment Neoxanthin Chlorophyta education 030304 developmental biology 0303 health sciences education.field_of_study biology Chlorophyll A Photosystem II Protein Complex Cell Biology Pigments Biological biology.organism_classification Siphonaxanthin chemistry SCP Siphonein visual_art visual_art.visual_art_medium 010606 plant biology & botany |
| Zdroj: | Biochimica Et Biophysica Acta. Bioenergetics Biochimica Et Biophysica Acta. Bioenergetics, 2021, 1862 (5), pp.148384. ⟨10.1016/j.bbabio.2021.148384⟩ |
| Popis: | The siphonaxanthin-siphonein-chlorophyll-a/b-binding protein (SCP), a trimeric light-harvesting complex isolated from photosystem II of the siphonous green alga Codium fragile, binds the carotenoid siphonaxanthin (Sx) and/or its ester siphonein in place of lutein, in addition to chlorophylls a/b and neoxanthin. SCP exhibits a higher content of chlorophyll b (Chl-b) than its counterpart in green plants, light-harvesting complex II (LHCII), increasing the relative absorption of blue-green light for photosynthesis. Using low temperature absorption and resonance Raman spectroscopies, we reveal the presence of two non-equivalent Sx molecules in SCP, and assign their absorption peaks at 501 and 535 nm. The red-absorbing Sx population exhibits a significant distortion that is reminiscent of lutein 2 in trimeric LHCII. Unexpected enhancement of the Raman modes of Chls-b in SCP allows an unequivocal description of seven to nine non-equivalent Chls-b, and six distinct Chl-a populations in this protein. |
| Databáze: | OpenAIRE |
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