Preparation of degradable porous structures based on 1,3-trimethylene carbonate and D,L-lactide (co)polymers for heart tissue engineering
| Autor: | J. Feijen, A.A. Poot, B. Siebum, Dirk W. Grijpma, M.J.A. van Luyn, X. J. Gallego y van Seijen, Ana Paula Pêgo |
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| Přispěvatelé: | Nanotechnology and Biophysics in Medicine (NANOBIOMED), Restoring Organ Function by Means of Regenerative Medicine (REGENERATE), Faculty of Science and Technology, Biomaterials Science and Technology |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Materials science
Biocompatible Materials chemistry.chemical_compound IR-67194 Copolymer Porosity chemistry.chemical_classification CELL-CULTURE EPSILON-CAPROLACTONE Tissue Engineering IN-VITRO DEGRADATION TRANSPLANTATION Myocardium TRIMETHYLENE CARBONATE BIOENGINEERED CARDIAC GRAFTS General Engineering Endothelial Cells SPONGES Polymer COPOLYMERS Transplantation POLY(L-LACTIC ACID) METIS-216593 chemistry Chemical engineering Microscopy Electron Scanning Molar mass distribution Particle size Trimethylene carbonate POLYMERS Porous medium |
| Zdroj: | Tissue Engineering, 9(5), 981-994. MARY ANN LIEBERT, INC Tissue engineering, 9(5), 981-994. Mary Ann Liebert Inc. |
| ISSN: | 1076-3279 |
| Popis: | Biodegradable porous scaffolds for heart tissue engineering were prepared from amorphous elastomeric (co)polymers of 1,3-trimethylene carbonate (TMC) and D,L-lactide (DLLA). Leaching of salt from compression-molded polymer-salt composites allowed the preparation of highly porous structures in a reproducible fashion. By adjusting the salt particle size and the polymer-to-particle weight ratio in the polymer-salt composite preparation the pore size and porosity of the scaffolds could be precisely controlled. The thermal properties of the polymers used for scaffold preparation had a strong effect on the morphology, mechanical properties and dimensional stability of the scaffolds under physiological conditions. Interconnected highly porous structures (porosity, 94%; average pore size, 100 microm) based on a TMC-DLLA copolymer (19:81, mol%) had suitable mechanical properties and displayed adequate cell-material interactions to serve as scaffolds for cardiac cells. This copolymer is noncytotoxic and allows the adhesion and proliferation of cardiomyocytes. During incubation in phosphate-buffered saline at 37 degrees C, these scaffolds were dimensionally stable and the number average molecular weight (Mn) of the polymer decreased gradually from 2.0 x 10(5) to 0.3 x 10(5) in a period up to 4 months. The first signs of mass loss (5%) were detected after 4 months of incubation. The degradation behavior of the porous structures was similar to that of nonporous films with similar composition and can be described by autocatalyzed bulk hydrolysis. |
| Databáze: | OpenAIRE |
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