Alterations in the Synthesis of a Fibroblast Surface Associated 35 K Protein Modulates the Binding of Somatomedin-C/lnsulin-Like Growth Factor I*
Autor: | V. K. M. Han, David R. Clemmons, A. Joseph D'Ercole, Robert G. Elgin |
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Rok vydání: | 1987 |
Předmět: |
medicine.medical_treatment
Cell Receptors Cell Surface Cycloheximide Biology chemistry.chemical_compound Endocrinology Somatomedins medicine Humans Secretion Insulin-Like Growth Factor I Receptor Fibroblast Molecular Biology Cells Cultured Growth factor Cell Membrane Receptors Somatomedin General Medicine Fibroblasts Molecular biology Somatomedin Molecular Weight Kinetics medicine.anatomical_structure chemistry Biochemistry Cell culture |
Zdroj: | Molecular Endocrinology. 1:339-347 |
ISSN: | 1944-9917 0888-8809 |
Popis: | Human fibroblasts, a cell type that is used extensively to determine the pleiotypic effects of the insulin-like growth factors, have been shown to secrete a 35K protein that binds somatomedin-C/insulin-like growth factor I (Sm-C/IGF-I) but not insulin. This 35 K protein is associated with the fibroblast surface and following transfer to the surface of cell types that do not have this protein on their surfaces, it alters the binding of radiolabeled Sm-C/IGF-I. In this study human fibroblast monolayers that were incubated with cyclohexamide (50 micrograms/ml) for 14 h at 37 C had no detectable 35 K protein on their cell surface, but type I Sm-C/IGF-I receptors were still present. Loss of the 35 K protein was associated with 60-70% increase in binding of Sm-C/IGF-I to type I receptors. The relative affinity of the type I receptor for Sm-C/IGF-I was apparently increased because unlabeled Sm-C/IGF-I (12 ng/ml) competitively displaced 63% of radiolabeled Sm-C/IGF-I after cycloheximide exposure, whereas in cultures not exposed to cycloheximide [125I]Sm-C/IGF-I binding was increased by 11%. Coincubation of fibroblast conditioned media containing the 35 K protein with cycloheximide-treated fibroblast monolayers resulted in restoration of the paradoxical increase in Sm-C/IGF-I binding and loss of sensitivity to competition by unlabeled Sm-C/IGF-I. Exposure of suspended fibroblasts, which do not have 35 K on their cell surface, to media conditioned by fibroblast monolayers also induced both of these changes.(ABSTRACT TRUNCATED AT 250 WORDS) |
Databáze: | OpenAIRE |
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