Molecular Viral Diagnosis and Sanitation of Yam Genetic Resources: Implications for Safe Yam Germplasm Exchange

Autor: Séverine Gallet, Claudie Pavis, Suzia Gélabale, Franciane Gamiette, Rose-Marie Gomez, Marie Umber, Pierre-Yves Teycheney, Denis Filloux, Armelle Marais
Přispěvatelé: Agrosystèmes tropicaux (ASTRO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), INRAE, the French government, the Guadeloupe Region and the European Union through Caramba (N 32920), CRBio (CRB PT 23092015) and Malin (2015-FED-187) projects
Rok vydání: 2020
Předmět:
0106 biological sciences
Germplasm
Sanitation
Collection botanique
[SDV]Life Sciences [q-bio]
lcsh:QR1-502
Polymerase Chain Reaction
01 natural sciences
lcsh:Microbiology
Plant Viruses
santé des plantes
Conservation du matériel génétique
Pathology
Molecular
pathologie végétale
West indies
2. Zero hunger
0303 health sciences
biology
Dioscorea
food and beverages
PCR
Infectious Diseases
virologie végétale
sanitation
West Indies
Article
Diagnostic de laboratoire
Crop
03 medical and health sciences
Genetic resources
Virology
molecular diagnosis
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
yam
viruses
germplasm conservation and dissemination
Tropical plants
Badnavirus
Plant Diseases
H20 - Maladies des plantes
030304 developmental biology
virus phytopathogène
Procédures opérationnelles normalisées d'assainissement
business.industry
Reproducibility of Results
Virus des végétaux
biology.organism_classification
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Biotechnology
Potexvirus
business
010606 plant biology & botany
Zdroj: Viruses
Volume 12
Issue 10
Viruses, Vol 12, Iss 1101, p 1101 (2020)
Viruses, MDPI, 2020, 12 (10), pp.1101. ⟨10.3390/v12101101⟩
ISSN: 1999-4915
DOI: 10.3390/v12101101
Popis: Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide.
Databáze: OpenAIRE
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