A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas
| Autor: | Zhongqiang Cai, Lei Wei, Wang Jiao, Cheng He, Xiaotong Wang, Jun Chen, Wen Guo, Qiuyun Jiang, Wu Changlu, Wenchao Yu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Oyster Physiology Biology 010603 evolutionary biology 01 natural sciences Genome lcsh:Physiology 03 medical and health sciences RNA interference Physiology (medical) biology.animal Mantle (mollusc) Gene Genetics lcsh:QP1-981 fungi adult oyster food and beverages Pacific oyster biology.organism_classification 030104 developmental biology Rhodopsin light-sensitivity RNAi biology.protein rhodopsin-like superfamily member gene Crassostrea mantle |
| Zdroj: | Frontiers in Physiology, Vol 9 (2018) |
| DOI: | 10.3389/fphys.2018.00221/full |
| Popis: | Light-sensitivity is important for mollusc survival, as it plays a vital role in reproduction and predator avoidance. Light-sensitivity has been demonstrated in the adult Pacific oyster Crassostrea gigas, but the genes associated with light-sensitivity remain unclear. In the present study, we designed experiments to identify the genes associated with light-sensitivity in adult oysters. First, we assessed the Pacific oyster genome and identified 368 genes annotated with the terms associated with light-sensitivity. Second, the function of the four rhodopsin-like superfamily member genes was tested by using RNAi. The results showed that the highest level of mRNA expression of the vision-related genes was in the mantle; however, this finding is not true for all oyster genes. Interestingly, we also found four rhodopsin-like superfamily member genes expressed at an very high level in the mantle tissue. In the RNAi experiment, when one of rhodopsin-like superfamily member genes (CGI_1001253) was inhibited, the light-sensitivity capacity of the injected oysters was significantly reduced, suggesting that CGI_10012534 may be associated with light-sensitivity in the adult Pacific oyster. |
| Databáze: | OpenAIRE |
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