Dissemination of carbapenem-resistant Acinetobacter baumannii in patients with burn injuries
| Autor: | Abbas Farahani, Mojtaba Moosavian, Soodabeh Rostami, Saeed Shoja, Amir Peymani, Khadijeh Ahmadi, Nasim Ebrahimifard |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Acinetobacter baumannii Male 030106 microbiology Antimicrobial susceptibility Microbial Sensitivity Tests Microbiology law.invention 03 medical and health sciences law Drug Resistance Bacterial polycyclic compounds medicine Humans In patient Polymerase chain reaction carbapenem- resistant Carbapenem resistance Medicine(all) lcsh:R5-920 biology General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses Virology Carbapenems Colistin bacteria Female Carbapenem resistant Acinetobacter baumannii lcsh:Medicine (General) Burns Polymyxin B medicine.drug |
| Zdroj: | Journal of the Chinese Medical Association, Vol 80, Iss 4, Pp 245-252 (2017) |
| ISSN: | 1728-7731 |
| Popis: | Background Carbapenem-resistant Acinetobacter baumannii has emerged as an important cause of infection in burn patients. This study aimed to characterize the antimicrobial susceptibility pattern, determine the prevalence of oxacillinase and metallo-beta-lactamase (MBL) genes, and type the A. baumannii isolates obtained from burn patients. Methods During a 1-year period, a total of 40 nonduplicated isolates of A. baumannii were obtained from burn patients who were hospitalized in the Taleghani Burn Hospital in Ahvaz, in the southwest of Iran. Testing for antimicrobial susceptibility was carried out by disk diffusion and E-test. To screen MBL production, a double disk synergy and MBL E-test were performed. The presence of bla OXA-23-like , bla OXA-24-like , bla OXA-51-like and bla OXA-58-like , bla VIM , bla IMP and bla SPM , and bla NDM was sought by polymerase chain reaction (PCR). Repetitive extragenic palindromic sequence-based PCR was carried out for determination of isolates clonality. Results Overall, 92.5% of isolates were carbapenem-resistant. Polymyxin B, colistin, and ampicillin-sulbactam were the most effective agents in vitro , with a susceptibility rate of 100%, 97.5%, and 72.5%, respectively. According to the double disk synergy and E-test, 55.6% and 97.3% of isolates were MBL producers, respectively. Furthermore, 70% of isolates harbored bla OXA-23-like and 20% were positive for bla OXA-24-like. However, no encoding genes were detected for bla VIM , bla IMP and bla SPM , bla NDM , and bla OXA-58-like . Repetitive extragenic palindromic sequence-based PCR revealed that carbapenem-resistant isolates belonged to four clones, including A, B, C, and D; the predominant clones were B and C. Conclusion The rate of carbapenem resistance was high, and it appeared that bla OXA-23-like and bla OXA-24-like contributed to the carbapenem resistance of A. baumannii isolates. This result suggests that the two predominant clones of A. baumannii were spread among burn patients. In order to prevent future dissemination of resistant isolates among burn patients, an effective infection control plan is necessary. |
| Databáze: | OpenAIRE |
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