Stimulation of transforming growth factor-beta 1 transcription by cyclosporine
| Autor: | Y. Prashar, Manikkam Suthanthiran, Ashwani Khanna, Prabodh K. Sehajpal, Vijay K. Sharma |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
TGF-β
Transcription Genetic medicine.medical_treatment T-Lymphocytes Molecular Sequence Data Biophysics Biology Biochemistry Cell Line Structural Biology Transcription (biology) Transforming Growth Factor beta CAT activity Genetics medicine Humans Electrophoretic mobility shift assay Promoter Regions Genetic Molecular Biology Transcription factor Mobility shift assay Base Sequence RNA Cell Biology Transfection Molecular biology DNA-Binding Proteins Cytokine Mechanism of action Oligodeoxyribonucleotides Cyclosporine medicine.symptom Transcription Transforming growth factor |
| Zdroj: | FEBS letters. 358(2) |
| ISSN: | 0014-5793 |
| Popis: | In searching for a candidate mechanism for the immunosuppressive as well as fibrogenic consequences of cyclosporine usage, we have explored the hypothesis that cyclosporine stimulates transcription of transforming growth factor- β 1 (TGF- β 1 ), a multifunctional cytokine endowed with immunosuppressive and fibrogenic properties. Our results demonstrate that cyclosporine (i) stimulates TGF- β 1 promoter-dependent transcription of chloramphenicol acetyl transferase gene in transiently transfected human A-549 cells, (ii) stimulates the synthesis of TGF- β 1 RNA transcripts in human T cells, and (iii) permits the expression/emergence of DNA regulatory proteins (retinoblastoma control factor-1 (RCF-1) and RCF-2) that bind and regulate TGF- β 1 promoter activity. Our studies demonstrate for the first time that cyclosporine stimulates TGF- β 1 gene transcription and suggest a novel mechanism of action of cyclosporine. |
| Databáze: | OpenAIRE |
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