Enhancement of the polycation-mediated DNA uptake and cell transfection with Pluronic P85 block copolymer
| Autor: | Irina V. Maksimova, Eugenii M. Lukanidin, Valery Yu Alakhov, Alexander V. Kabanov, Irina Astafieva |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Calcium Phosphates
Chloramphenicol O-Acetyltransferase Biophysics Pluronic copolymer Polypropylenes Transfection Biochemistry Jurkat cells Plasmid 3T3 cells Cell Line Polyethylene Glycols Polycation Chloramphenicol acetyltransferase chemistry.chemical_compound Mice Structural Biology Genes Reporter Surfactant Genetics medicine Polyamines Animals Humans Molecular Biology Micelles Ethylene oxide Gene Transfer Techniques Temperature 3T3 cell Cell Biology 3T3 Cells DNA Molecular biology Polyelectrolytes Endocytosis medicine.anatomical_structure chemistry Cell culture Poloxalene Polyvinyls Plasmids |
| Zdroj: | FEBS Letters. (3):278-280 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(96)00607-2 |
| Popis: | Polyelectrolyte complexes formed between DNA and poly(N-ethyl-4-vinylpyridinium) cations were shown to effectively transfect mammalian cells [7]. This work suggests that the polycation-mediated uptake of the plasmid DNA and cell transfection are significantly enhanced when these complexes are administered simultaneously with a poly(ethylene oxide)block-poly(propylene oxide)-block-poly(ethylene oxide) copolymer, Pluronic P85. The uptake studies were performed using radioactively labeled pRSV CAT plasmid on NIH 3T3, MDCK, and Jurkat cell lines. The transfection was investigated by chloramphenicol acetyltransferase assay using 3T3 cells as a model. The effects reported may be useful for the enhancement of the polycation-mediated cell transfection. |
| Databáze: | OpenAIRE |
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