Protection Against Preterm Delivery in Mice by Urinary Trypsin Inhibitor
| Autor: | Yasunori Shibutani, Nobuhiko Kaga, Yoshihiro Futamura, Satoshi Kajikawa |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Lipopolysaccharide medicine.medical_treatment Proinflammatory cytokine chemistry.chemical_compound Mice Obstetric Labor Premature In vivo Pregnancy Internal medicine Medicine Animals Receptor Glycoproteins Mice Inbred C3H business.industry Tumor Necrosis Factor-alpha Macrophages Obstetrics and Gynecology In vitro Intracellular signal transduction Cytokine Endocrinology chemistry Cytokines Tumor necrosis factor alpha Female business Trypsin Inhibitors |
| Zdroj: | Obstetrics & Gynecology. 93:100-108 |
| ISSN: | 0029-7844 |
| DOI: | 10.1097/00006250-199901000-00021 |
| Popis: | Objective: To investigate the precise mechanism by which urinary trypsin inhibitor suppresses cytokine production in the prevention of preterm delivery. Methods: In vivo and in vitro studies were performed using ascites and peritoneal macrophages obtained on day 15 of pregnancy from female C3H/HeN mice that had been impregnated by B6D2F1 male mice. Lipopolysaccharide receptor, the intracellular signal transduction system, and nuclear factor-κB level were examined. Results: In the in vivo study, we found that urinary trypsin inhibitor ameliorated the deterioration of intraperitoneal conditions induced by lipopolysaccharide (ie, increases in ascitic volume, peritoneal cell count, and tumor necrosis factor-α level) and caused a decrease in the binding of lipopolysaccharide to mouse macrophages. In the in vitro studies, urinary trypsin inhibitor decreased the binding capacity of lipopolysaccharide for its receptor, blocked the intracellular signal transduction induced by lipopolysaccharide, and decreased the nuclear factor-κB level. Increases were induced in the binding capacity of the macrophages for urinary trypsin inhibitor and its incorporation into them in the presence of lipopolysaccharide. Conclusion: We postulate that urinary trypsin inhibitor may suppress the production of inflammatory cytokines induced by lipopolysaccharide in mouse peritoneal macrophages through suppression of the lipopolysaccharide receptor, inhibition of the intracellular signal transduction system, and decrease in the nuclear factor-κB level. |
| Databáze: | OpenAIRE |
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