AMPA receptor expression in mouse testis and spermatogonial GC-1 cells: A study on its regulation by excitatory amino acids
| Autor: | Alessandra Santillo, Paolo Chieffi, Federica Di Giacomo Russo, Sara Falvo, Maria Maddalena Di Fiore, Alessandro Usiello, Claudia Pinelli, Gabriella Chieffi Baccari |
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| Přispěvatelé: | Santillo, Alessandra, Falvo, Sara, Di Fiore, Maria Maddalena, Di Giacomo Russo, Federica, Chieffi, Paolo, Usiello, Alessandro, Pinelli, Claudia, Chieffi Baccari, Gabriella |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway AMPA receptor Biochemistry proliferating cell nuclear antigen (PCNA) 03 medical and health sciences 0302 clinical medicine Aurora B Receptor Molecular Biology Protein kinase B biology Kinase Chemistry Akt Glutamate receptor excitatory amino acid Cell Biology Proliferating cell nuclear antigen Cell biology spermatogonia 030104 developmental biology extracellular signal-regulated kinase (ERK) 030220 oncology & carcinogenesis biology.protein NMDA receptor |
| Popis: | Excitatory amino acids (EAAs) are found present in the nervous and reproductive systems of animals. Numerous studies have demonstrated a regulatory role for Glutamate (Glu), d-aspartate ( d-Asp) and N-methyl- d-aspartate (NMDA) in the control of spermatogenesis. EAAs are able to stimulate the Glutamate receptors, including the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR). Here in, we assess expression of the main AMPAR subunits, GluA1 and GluA2/3, in the mouse testis and in spermatogonial GC-1 cells. The results showed that both GluA1 and GluA2/3 were localized in mouse testis prevalently in spermatogonia. The subunit GluA2/3 was more highly expressed compared with GluA1 in both the testis and the GC-1 cells. Subsequently, GC-1 cells were incubated with medium containing l-Glu, d-Glu, d-Asp or NMDA to determine GluA1 and GluA2/3 expressions. At 30 minutes and 2 hours of incubation, EAA-treated GC-1 cells showed significantly higher expression levels of both GluA1 and GluA2/3. Furthermore, p-extracellular signal-regulated kinase (ERK), p-Akt, proliferating cell nuclear antigen (PCNA), and Aurora B expressions were assayed in l-Glu-, d-Glu-, and NMDA-treated GC-1 cells. At 30 minutes and 2 hours of incubation, treated GC-1 cells showed significantly higher expression levels of p-ERK and p-Akt. A consequent increase of PCNA and Aurora B expressions was induced by l-Glu and NMDA, but not by d-Glu. Our study demonstrates a direct effect of the EAAs on spermatogonial activity. In addition, the increased protein expression levels of GluA1 and GluA2/3 in EAA-treated GC-1 cells suggest that EAAs could activate ERK and Akt pathways through the AMPAR. Finally, the increased PCNA and Aurora B levels may imply an enhanced proliferative activity. |
| Databáze: | OpenAIRE |
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