Isolation, culture and division of olive (Olea europaea L.) protoplasts
| Autor: | Luis A. Cañas, M C Benbadis, A M Wyssmann |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
food.ingredient
biology Cell division fungi food and beverages Plant Science General Medicine biochemical phenomena metabolism and nutrition Protoplast biology.organism_classification chemistry.chemical_compound food chemistry Cell culture Olea Callus Botany Agarose Centrifugation Agronomy and Crop Science Cotyledon |
| Zdroj: | Plant cell reports. 6(5) |
| ISSN: | 0721-7714 |
| Popis: | Protoplasts from Olea europaea L. have been compared in terms of their yield, viability, cell division and callus differentiation. Viable protoplasts were isolated from in vitro cultured leaves and cotyledons by an overnight incubation in an enzyme solution containing 1-1.5% driselase and 0.5M sucrose. This method allowed high yield of purified protoplasts, which floated and formed a dark green band at the meniscus, after centrifugation. Purified protoplasts were diluted to 3×10(4) protoplasts·ml(-1) in culture medium. After cell wall regeneration, protoplasts gradually increased their volumes under appropriate conditions. The first divisions occurred during the second week in culture. Division efficiency ranged from 5.2 to 9.8% after 20 days in culture. Two weeks later visible microcolonies developed only from cotyledon protoplasts. After 6 weeks in culture, the microcalli were transferred to a solidified culture medium with 0.6% agarose, which induced active callus growth. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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