De Novo Acquisition of Resistance to SCY-078 in Candida glabrata Involves FKS Mutations That both Overlap and Are Distinct from Those Conferring Echinocandin Resistance
| Autor: | Katyna Borroto-Esoda, David S. Perlin, Cristina Jiménez-Ortigosa, Winder B. Perez, David Angulo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pharmacology Genetics Candida glabrata biology Echinocandin ATP synthase Chemistry 030106 microbiology Broth microdilution biology.organism_classification Molecular biology In vitro 03 medical and health sciences 030104 developmental biology Infectious Diseases Mechanisms of Resistance medicine biology.protein Pharmacology (medical) Binding site Echinocandins IC50 medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 61 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.00833-17 |
| Popis: | SCY-078 is an orally active antifungal whose target is the β-(1,3)- d -glucan synthase (GS). We evaluated the spontaneous emergence of SCY-078-resistant Candida glabrata isolates following drug exposure in vitro . Resistant isolates were analyzed using broth microdilution methodology and FKS sequencing. The kinetic inhibition parameter IC 50 (50% inhibitory concentration) was also determined from GS complexes. The spectrum of resistance mutations found suggested a partially overlapping but independent binding site for SCY-078 relative to echinocandins on GS. |
| Databáze: | OpenAIRE |
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