Study of changes in diacylglycerol content on nerve excitation
| Autor: | I. P. Gruniushkin, Georgy V. Maksimov, M. A. Yudanov, Elvira Sergeevna Revina, Victor V. Revin |
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| Rok vydání: | 2006 |
| Předmět: |
Phosphatidylinositol 4
5-Diphosphate PLCD3 Rhythmic excitation PLCB2 Biology Biochemistry Membrane Potentials Diglycerides chemistry.chemical_compound Phosphatidylinositol Phosphates Phosphoinositide phospholipase C Animals Phosphatidylinositol Enzyme Inhibitors Protein Kinase C Protein kinase C Diacylglycerol kinase Phospholipase C Biological Transport General Medicine Sciatic Nerve Phenylmethylsulfonyl Fluoride chemistry Biophysics Calcium lipids (amino acids peptides and proteins) Rabbits |
| Zdroj: | Biochemistry (Moscow). 71:1096-1100 |
| ISSN: | 1608-3040 0006-2979 |
| DOI: | 10.1134/s0006297906100063 |
| Popis: | Rhythmic excitation of a rabbit myelin nerve increased diacylglycerol (DAG) content from 1.53 to 2.17 microg/mg lipids. Inhibition of phosphoinositide-specific phospholipase C decreased DAG content. This suggests involvement of this enzyme in processes accompanying rhythmic excitation. The increase in membrane potential of the nerve fiber (K+-depolarization) was accompanied by increase in DAG and phosphatidylinositol monophosphate and decrease in phosphatidylinositol triphosphate and phosphatidylinositol diphosphate content. Treatment of the nerve with DAG or a protein kinase C activator increased (45)Ca influx by 40%, whereas treatment with an inhibitor of this enzyme, polymyxin, inhibited this parameter by 34%. The role of phosphoinositides and protein kinase C in the regulation of Ca2+ transport during rhythmic excitation of the myelin nerve is discussed. |
| Databáze: | OpenAIRE |
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