Prenatal ethanol exposure impairs the formation of radial glial fibers and promotes the transformation of GFAPδ‑positive radial glial cells into astrocytes
| Autor: | Hong Zhang, Yu Li, Feng-Yu Xi, Yue Liu, Lina Zhang, Xiang-Long Duan, Li Chong |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research prenatal ethanol exposure Neurogenesis Ependymoglial Cells radial glial cells Vimentin Biochemistry Nestin 03 medical and health sciences 0302 clinical medicine Pregnancy Glial Fibrillary Acidic Protein embryonic cerebral cortex Genetics Animals radial glial fiber glial fibrillary acidic protein δ Intermediate filament Molecular Biology Mitosis Cell Proliferation Oncogene Glial fibrillary acidic protein biology Ethanol Chemistry Brain Articles Cell cycle Embryonic stem cell Cell biology Mice Inbred C57BL 030104 developmental biology Oncology nervous system Maternal Exposure 030220 oncology & carcinogenesis Astrocytes biology.protein Molecular Medicine Female |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | During embryonic cortical development, radial glial cells (RGCs) are the major source of neurons, and these also serve as a supportive scaffold to guide neuronal migration. Similar to Vimentin, glial fibrillary acidic protein (GFAP) is one of the major intermediate filament proteins present in glial cells. Previous studies confirmed that prenatal ethanol exposure (PEE) significantly affected the levels of GFAP and increased the disassembly of radial glial fibers. GFAPδ is a variant of GFAP that is specifically expressed in RGCs; however, to the best of our knowledge, there are no reports regarding how PEE influences its expression during cortical development. In the present study, the effects of PEE on the expression and distribution of GFAPδ during early cortical development were assessed. It was found that PEE significantly decreased the expression levels of GFAP and GFAPδ. Using double immunostaining, GFAPδ was identified to be specifically expressed in apical and basal RGCs, and was co‑localized with other intermediate filament proteins, such as GFAP, Nestin and Vimentin. Additionally, PEE significantly affected the morphology of radial glial fibers and altered the behavior of RGCs. The loss of GFAPδ accelerated the transformation of RGCs into astrocytes. Using co‑immunostaining with Ki67 or phospho‑histone H3, GFAPδ+ cells were observed to be proliferative or mitotic cells, and ethanol treatment significantly decreased the proliferative or mitotic activities of GFAPδ+ RGCs. Taken together, the results suggested that PEE altered the expression patterns of GFAPδ and impaired the development of radial glial fibers and RGC behavior. The results of the present study provided evidence that GFAPδ may be a promising target to rescue the damage induced by PEE. |
| Databáze: | OpenAIRE |
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