Pregnancy does not adversely impact diagnostic tests for HTLV-1/2 infection
| Autor: | Jennifer Tosswill, Richard S. Tedder, Carolina Rosadas, Graham P. Taylor |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
RNA viruses Physiology Maternal Health viruses Artificial Gene Amplification and Extension Pathology and Laboratory Medicine Biochemistry Polymerase Chain Reaction law.invention White Blood Cells 0302 clinical medicine law Pregnancy Animal Cells immune system diseases Immune Physiology hemic and lymphatic diseases Medicine and Health Sciences Enzyme-Linked Immunoassays Polymerase chain reaction 11 Medical and Health Sciences Immunoassay Human T-lymphotropic virus 1 Serodiagnosis Immune System Proteins biology T Cells lcsh:Public aspects of medicine Human T-lymphotropic virus 2 Diagnostic test Obstetrics and Gynecology virus diseases Viral Load Serology Infectious Diseases Medical Microbiology Viral Pathogens Viruses Female Antibody Pathogens Cellular Types Viral load Research Article medicine.medical_specialty lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Immune Cells 030231 tropical medicine Immunology Sexually Transmitted Diseases Real-Time Polymerase Chain Reaction Research and Analysis Methods Microbiology Virus Antibodies 03 medical and health sciences Paired samples Diagnostic Medicine Tropical Medicine Retroviruses medicine Humans Immunoassays Molecular Biology Techniques Microbial Pathogens Molecular Biology Blood Cells Biology and life sciences business.industry Public Health Environmental and Occupational Health Organisms Proteins lcsh:RA1-1270 Htlv-1 Cell Biology 06 Biological Sciences medicine.disease HTLV-I Infections Pregnancy Complications 030104 developmental biology Tropical medicine HTLV-II Infections biology.protein Immunologic Techniques Women's Health business |
| Zdroj: | PLoS Neglected Tropical Diseases, Vol 13, Iss 9, p e0007736 (2019) PLoS Neglected Tropical Diseases |
| ISSN: | 1935-2735 1935-2727 |
| Popis: | Mother-to-child-transmission (MTCT) of human T-cell lymphotropic virus type-1(HTLV-1) contributes disproportionately to the burden of HTLV-1 associated diseases. All preventive measures to avoid MTCT rely on the identification of infected mothers. However, the impact of pregnancy on HTLV-1 diagnosis has not been clearly assessed. Paired samples from 21 HTLV-1 infected women taken during pregnancy and while not pregnant were analysed by CMIA and PCR. The signal-to-cut-off values (S/CO) were higher during pregnancy than in the paired non-pregnant samples. HTLV-1 proviral load did not alter significantly by pregnant state. S/CO positively correlated with HTLV proviral load. Pregnancy does not impair the diagnosis of HTLV-1/2, by either immunological (CMIA) or molecular (qPCR/nPCR) tests. Author summary Human T-cell lymphotropic virus (HTLV) can be transmitted from mother to child, mainly by breastfeeding. All preventive measures to avoid HTLV mother to child transmission depend on the identification of infected pregnant women. HTLV diagnosis is based on serological screening tests, confirmed by serology and/or molecular assays. The aim of the study was to verify whether pregnancy adversely impacts the identification of HTLV infection. Using paired samples from 21 women living with HTLV-1/2 obtained during pregnancy and while not pregnant we demonstrate that both serology and molecular assays perform equally well in both settings and can be use for the diagnosis of HTLV infection during pregnancy. |
| Databáze: | OpenAIRE |
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