Molecular biology interventions for activity improvement and production of industrial enzymes
| Autor: | Gopalakrishnan Kumar, Meenu Thakur, Vinod Kumar, Dinesh Kumar, Yung Hun Yang, Narisetty Vivek, Arivalagan Pugazendhi, Neha Chandel, Shashi Kant Bhatia |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Environmental Engineering Process (engineering) Computer science Bioengineering 010501 environmental sciences Protein Engineering 01 natural sciences Catalysis Substrate Specificity Metabolic engineering 010608 biotechnology Industry Production (economics) Cosmetic Molecular Biology Waste Management and Disposal 0105 earth and related environmental sciences Renewable Energy Sustainability and the Environment Industrial scale General Medicine Directed evolution Molecular biology Surface display Enzymes Industrial enzymes Metagenomics Enzyme Pharmaceutical Directed Molecular Evolution Fusion protein |
| Popis: | Metagenomics and directed evolution technology have brought a revolution in search of novel enzymes from extreme environment and improvement of existing enzymes and tuning them towards certain desired properties. Using advanced tools of molecular biology i.e. next generation sequencing, site directed mutagenesis, fusion protein, surface display, etc. now researchers can engineer enzymes for improved activity, stability, and substrate specificity to meet the industrial demand. Although many enzymatic processes have been developed up to industrial scale, still there is a need to overcome limitations of maintaining activity during the catalytic process. In this article recent developments in enzymes industrial applications and advancements in metabolic engineering approaches to improve enzymes efficacy and production are reviewed. |
| Databáze: | OpenAIRE |
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