Inhibition of PACT-Mediated Activation of PKR by the Herpes Simplex Virus Type 1 Us11 Protein
Autor: | David Khoo, Ganes C. Sen, Ian Mohr, Gregory A. Peters |
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Rok vydání: | 2002 |
Předmět: |
Viral protein
viruses Immunology Apoptosis RNA-binding protein Herpesvirus 1 Human Biology medicine.disease_cause Pact environment and public health Microbiology Viral Proteins eIF-2 Kinase Enzyme activator Virology Tumor Cells Cultured medicine Humans EIF-2 kinase integumentary system Activator (genetics) RNA-Binding Proteins virus diseases biochemical phenomena metabolism and nutrition Molecular biology Protein kinase R Virus-Cell Interactions Enzyme Activation Herpes simplex virus Insect Science biology.protein |
Zdroj: | Journal of Virology. 76:11054-11064 |
ISSN: | 1098-5514 0022-538X |
Popis: | PACT, a protein activator of PKR, can cause inhibition of cellular protein synthesis and apoptosis. Here, we report that the Us11 protein of herpes simplex virus type 1 can block PKR activation by PACT both in vitro and in vivo. Although Us11 can bind to both PKR and PACT, mutational analyses revealed that the binding of Us11 to PKR, and not to PACT, was essential for its inhibitory action. Similar analyses also revealed that the inhibitory effect was mediated by an interaction between the C-terminal half of Us11 and the N-terminal domain of PKR. The binding of Us11 to PKR did not block the binding of PKR to PACT but prevented its activation. Us11 is the first example of a viral protein that can inhibit the action of PACT on PKR. |
Databáze: | OpenAIRE |
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