Controlling CRISPR‐Cas9 by guide RNA engineering

Autor: Lu‐Feng Hu, Yu‐Xuan Li, Jia‐Zhen Wang, Yu‐Ting Zhao, Yangming Wang
Rok vydání: 2022
Předmět:
Zdroj: WIREs RNA. 14
ISSN: 1757-7012
1757-7004
DOI: 10.1002/wrna.1731
Popis: The clustered regularly interspaced short palindromic repeats (CRISPR) system is a product of million years of evolution by microbes to fight against invading genetic materials. Around 10 years ago, scientists started to repurpose the CRISPR as genetic tools by molecular engineering approaches. The guide RNA provides a versatile and unique platform for the innovation to improve and expand the application of CRISPR-Cas9 system. In this review, we will first introduce the basic sequence and structure of guide RNA and its role during the function of CRISPR-Cas9. We will then summarize recent progress on the development of various guide RNA engineering strategies. These strategies have been dedicated to improve the performance of CRISPR-Cas9, to achieve precise spatiotemporal control of CRISPR-Cas9, and to broaden the application of CRISPR-Cas9. Finally, we will briefly discuss the uniqueness and advantage of guide RNA-engineering based systems versus those with engineered Cas9 proteins and speculate potential future directions in guide RNA engineering. This article is categorized under: RNA MethodsRNA Analyses In Vitro and In Silico RNA MethodsRNA Nanotechnology Regulatory RNAs/RNAi/RiboswitchesRegulatory RNAs RNA Interactions with Proteins and Other MoleculesRNA-Protein Complexes.
Databáze: OpenAIRE
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