Thyroid disrupting effects of low-dose dibenzothiophene and cadmium in single or concurrent exposure: New evidence from a translational zebrafish model
| Autor: | Marco Borsò, Letizia Pitto, Grazia Rutigliano, M.S. Milella, F. Forini, Francesca Gorini, Elena Guzzolino, Giorgio Iervasi, Riccardo Zucchi, Alessandro Saba, Fabrizio Bianchi |
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| Rok vydání: | 2020 |
| Předmět: |
Thyroid Hormones
Environmental Engineering 010504 meteorology & atmospheric sciences Transgene thyroid hormone receptors Thyroid Gland Thiophenes 010501 environmental sciences Endocrine Disruptors 01 natural sciences Energy homeostasis In vivo medicine Environmental Chemistry Endocrine system Animals Humans dibenzothiophene Waste Management and Disposal Zebrafish 0105 earth and related environmental sciences Thyroid hormone receptor biology Chemistry cadmium zebrafish embryos hypothalamic-pituitary-thyroid axis disruption cardiac defects Thyroid biology.organism_classification Pollution Hypothalamic–pituitary–thyroid axis Cell biology medicine.anatomical_structure Water Pollutants Chemical Cadmium |
| Zdroj: | The Science of the total environment. 769 |
| ISSN: | 1879-1026 |
| Popis: | Thyroid hormones (THs) are major regulators of biological processes essential for correct development and energy homeostasis. Although thyroid disruptors can deeply affect human health, the impact of exogenous chemicals and in particular mixture of chemicals on different aspects of thyroid development and metabolism is not yet fully understood. In this study we have used the highly versatile zebrafish model to assess the thyroid axis disrupting effects of cadmium (Cd) and dibenzothiophene (DBT), two environmental endocrine disruptors found to be significantly correlated in epidemiological co-exposure studies. Zebrafish embryos (5hpf) were exposed to low concentrations of Cd (from 0.05 to 2 μM) and DBT (from 0.05 to 1 μM) and to mixtures of them. A multilevel assessment of the pollutant effects has been obtained by combining in vivo morphological analyses allowed by the use of transgenic fluorescent lines with liquid chromatography mass spectrometry determination of TH levels and quantification of the expression levels of key genes involved in the Hypothalamic-Pituitary-Thyroid Axis (HPTA) and TH metabolism. Our results underscore for the first time an important synergistic toxic effect of these pollutants on embryonic development and thyroid morphology highlighting differences in the mechanisms through which they can adversely impact on multiple physiological processes of the HPTA and TH disposal influencing also heart geometry and function. |
| Databáze: | OpenAIRE |
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