PCDAmpl, a new antigen at the interface of the embryonic collecting duct epithelium and the nephrogenic mesenchyme
| Autor: | Raimund Strehl, Sabine Kloth, Will W. Minuth, Pat Steiner, Joachim Aigner |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Basement membrane
medicine.medical_specialty Mesenchyme Nephron Biology Cholangiocyte Epithelium collecting duct Cell biology antigen Endocrinology medicine.anatomical_structure Nephrology Laminin Internal medicine medicine biology.protein monoclonal antibodies perfusion culture development Duct (anatomy) Fetal bovine serum |
| Zdroj: | Kidney International. 52:1469-1477 |
| ISSN: | 0085-2538 |
| DOI: | 10.1038/ki.1997.477 |
| Popis: | P CD Ampl, a new antigen at the interface of the embryonic collecting duct epithelium and the nephrogenic mesenchyme. In the neonatal rabbit kidney nephrogenesis is not yet terminated. The ampullar collecting duct epithelium acts as an inducer that generates the nephron anlagen, however, to date the morphogenic mechanisms involved are unknown. A presupposition for successful nephron induction is the close tissue interaction between the basal aspect of the ampullar collecting duct epithelium and the surrounding mesenchyme. To gain new insights in this area we raised monoclonal antibodies (mabs), to identify specific structures localized at the tissue interface. With the generated mab CD Ampl we found an intensive immunohistochemical reaction between the basal aspect of the ampullar collecting duct epithelium and the mesenchyme. The label was most concentrated at the ampullar tip and continuously decreased in the shaft region. In the maturing collecting duct of the neonatal kidney and in the adult renal collecting duct no immunohistochemical reaction was found. The binding pattern of mab CD Ampl is different from that of all known collecting duct cell markers and from antibodies against known basement membrane compounds such as laminin or collagen type IV. Under in vitro conditions immunoreactivity with mab CD Ampl was obtained using embryonic collecting duct epithelia and perfusion culture. The antigen was present in specimens treated with Iscove's modified Dulbecco's Medium (IMDM) containing 10% fetal bovine serum. Omittance of serum or hormonal treatment with aldosterone, insulin or vitamin D 3 led to the disappearance of the newly detected antigen, while characteristics of the differentiated collecting duct cells were up-regulated. We conclude that the expression of P CD Ampl is a characteristic feature of the embryonic parts of the collecting duct epithelium. It may play a pivotal role during nephron induction. |
| Databáze: | OpenAIRE |
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