Interleukin-23 Increases Intestinal Epithelial Cell Permeability In Vitro
| Autor: | Katherine Fredrich, Jennifer L. Liedel, Deborah L. Donohoe, David M. Gourlay, Nathan P. Heinzerling |
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| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Membrane Permeability Breast milk Real-Time Polymerase Chain Reaction Occludin Interleukin-23 Cell Line Proinflammatory cytokine Andrology Intestine Small Animals Humans Medicine RNA Messenger skin and connective tissue diseases Barrier function Milk Human Tight junction business.industry Dextrans Epithelial Cells Epithelium Rats Staining medicine.anatomical_structure Cell culture Pediatrics Perinatology and Child Health Zonula Occludens-1 Protein Female Surgery business Fluorescein-5-isothiocyanate |
| Zdroj: | European Journal of Pediatric Surgery. 26:260-266 |
| ISSN: | 1439-359X 0939-7248 |
| DOI: | 10.1055/s-0035-1551563 |
| Popis: | Background Breast milk has a heterogeneous composition that differs between mothers and changes throughout the first weeks after birth. The proinflammatory cytokine IL-23 has a highly variable expression in human breast milk. We hypothesize that IL-23 found in human breast milk is biologically active and promotes epithelial barrier dysfunction. Methods The immature rat small intestinal epithelial cell line, IEC-18, was grown on cell inserts or standard cell culture plates. Confluent cultures were exposed to human breast milk with high or low levels of IL-23 and barrier function was measured using a flux of fluorescein isothiocyanate–dextran (FD-70). In addition, protein and mRNA expression of occludin and ZO-1 were measured and immunofluorescence used to stain occludin and ZO-1. Results Exposure to breast milk with high levels of IL-23 caused an increase flux of FD-70 compared with both controls and breast milk with low levels of IL-23. The protein expression of ZO-1 but not occludin was decreased by exposure to high levels of IL-23. These results correlate with immunofluorescent staining of ZO-1 and occludin which show decreased staining of occludin in both the groups exposed to breast milk with high and low IL-23. Conversely, cells exposed to high IL-23 breast milk had little peripheral staining of ZO-1 compared with controls and low IL-23 breast milk. Conclusion IL-23 in human breast milk is biologically active and negatively affects the barrier function of intestinal epithelial cells through the degradation of tight junction proteins. |
| Databáze: | OpenAIRE |
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