Low expression of p27Kip1 in advanced mucoepidermoid carcinomas of head and neck

Autor: Choong Sik Choi, Geon Choi, Yang Seok Chae, Kwang Yoon Jung, Jong Ouck Choi
Rok vydání: 2001
Předmět:
Zdroj: Head & Neck. 23:292-297
ISSN: 1097-0347
1043-3074
DOI: 10.1002/hed.1033
Popis: Background p27Kip1, a cyclin-dependent kinase inhibitor, negatively regulates the G1 phase progression of the cell cycle by binding to the cyclin E/cyclin-dependent kinase 2 complex. This study was done to investigate the expression of p27Kip1 in mucoepidermoid carcinomas and its usefulness as an indicator in tumor progression, aggressiveness, and prognosis. Methods Thirty-one patients with mucoepidermoid carcinomas who had surgical resection were studied retrospectively. Clinicopathologic features, including histologic types, T stage, nodal status, perineural invasion, overall AJCC stage, and survival data, were obtained from medical records. Immunohistochemical staining with monoclonal antibodies against p27Kip1 was performed on the formalin-fixed, paraffin-embedded specimens from each patient. The percentage of tumor cells expressing p27Kip1 (labeling index) was evaluated by counting 1000 cells per slide in at least four different areas and comparing with the patients' clinicopathologic features and survival rates. Results Significant correlation was found between low p27Kip1 expression and tumors with high-grade, advanced T stages, positive nodal status, and advanced clinical stages (p = .001 for all) except perineural invasion. Multivariate analysis indicated that p27Kip1 expression (p = .030) was the most significant, and gender (p = .048) was the next significant predictor of overall survival among the variables. Also patients with low p27Kip1 expression showed poor prognosis (p = .002). Conclusions We suggest that p27Kip1 is a reliable independent marker of tumor progression, invasiveness, and prognosis in the mucoepidermoid carcinomas. © 2001 John Wiley & Sons, Inc. Head Neck 22: 292–297, 2001.
Databáze: OpenAIRE
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