Mucosal delivery of murine interleukin-2 (IL-2) and IL-6 by recombinant of Lactococcus lactis coexpressing antigen and cytokine
| Autor: | Richard W.F. Le Page, Jeremy Mark Wells, Erik Remaut, Karen Robinson, Karin Schofield, Lothar Steidler, L. M. Chamberlain |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Interleukin 2
medicine.medical_treatment Immunology Biology Microbiology Mice Immune system Antigen Tetanus Toxin medicine Animals Life Science Administration Intranasal Recombination Genetic Interleukin-6 Immunogenicity Lactococcus lactis biology.organism_classification Virology Antibodies Bacterial Peptide Fragments Immunoglobulin A Bacterial vaccine Mice Inbred C57BL Infectious Diseases Microbial Immunity and Vaccines biology.protein Interleukin-2 Parasitology Female Immunization Antibody Adjuvant medicine.drug |
| Zdroj: | Infection and Immunity 66 (1998) 7 Infection and Immunity, 66(7), 3183-3189 |
| ISSN: | 0019-9567 |
| Popis: | Lactococcus lactis is a nonpathogenic and noncolonizing bacterium which is being developed as a vaccine delivery vehicle for immunization by mucosal routes. To determine whether lactococci can also deliver cytokines to the immune system, we have constructed novel constitutive expression strains of L. lactis which accumulate a test antigen, tetanus toxin fragment C (TTFC), within the cytoplasmic compartment and also secrete either murine interleukin-2 (IL-2) or IL-6. When mice were immunized intranasally with various different expression strains of L. lactis , the anti-TTFC antibody titers increased more rapidly and were substantially higher in mice immunized with the bacterial strains which secreted IL-2 or IL-6 in addition to their production of TTFC. This adjuvant effect was lost when the recombinant strains of L. lactis were killed by pretreatment with mitomycin C and could therefore be attributed to the secretion of IL-2 or IL-6 by the recombinant lactococci. These results provide the first example of the use of a cytokine-secreting, noninvasive experimental bacterial vaccine vector to enhance immune responses to a coexpressed heterologous antigen and point the way to experiments which will test the possible therapeutic efficacy of this mode of cytokine delivery. |
| Databáze: | OpenAIRE |
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