Identification of powdery mildew resistance QTL in strawberry (Fragaria × ananassa)

Autor: Kirsty J. McLeary, Daniel J. Sargent, Maria Lumbreras-Martinez, Robert J. Vickerstaff, Laima Antanaviciute, Amanda Karlström, Katalin Pakozdi, Andy J. Passey, Helen M. Cockerton, Maria K. Sobczyk, Joe Q. He, Fiona M. Wilson, Richard J. Harrison, D. W. Simpson, Nicola Harrison
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Theoretical and Applied Genetics 131 (2018) 9
TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
Theoretical and Applied Genetics, 131(9), 1995-2007
ISSN: 0040-5752
1995-2007
Popis: Key Message Powdery mildew resistance in two strawberry mapping populations is controlled by both stable and transient novel QTL of moderate effect. Some low transferability of QTL across wider germplasm was observed. Abstract The obligate biotrophic fungus Podosphaera aphanis is the causative agent of powdery mildew on cultivated strawberry (Fragaria × ananassa). Genotypes from two bi-parental mapping populations ‘Emily’ × ‘Fenella’ and ‘Redgauntlet’ × ‘Hapil’ were phenotyped for powdery mildew disease severity in a series of field trials. Here, we report multiple QTL associated with resistance to powdery mildew, identified in ten phenotyping events conducted across different years and locations. Six QTL show a level of stable resistance across multiple phenotyping events; however, many other QTL were represented in a single phenotyping event and therefore must be considered transient. Subsequent screening of identified QTL across a validation set determined whether identified QTL remained closely linked to the associated resistance gene in the wider germplasm. Furthermore, a preliminary association analysis identified a novel conserved locus for further investigation. Our data suggest that resistance is highly complex and that multiple, primarily additive, sources of quantitative resistance to powdery mildew exist across strawberry germplasm. Utilisation of the reported markers in marker-assisted breeding or genomic selection would lead to improved powdery mildew-resistant strawberry cultivars, particularly where the studied parents, progeny and close pedigree material are included in breeding germplasm. Electronic supplementary material The online version of this article (10.1007/s00122-018-3128-0) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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