TGF-beta expression during rat pregnancy and activity on decidual cell survival

Autor: Carl Shooner, Eric Asselin, Marie-Claude Dery, Valérie Leblanc, Guylaine Fréchette-Frigon, Pierre-Luc Caron
Rok vydání: 2005
Předmět:
Apoptosis
Smad2 Protein
Endometrium
Rats
Sprague-Dawley
0302 clinical medicine
Endocrinology
Pregnancy
Transforming Growth Factor beta
lcsh:Reproduction
Decidual cells
Phosphorylation
Cells
Cultured
0303 health sciences
030219 obstetrics & reproductive medicine
Decidua
Obstetrics and Gynecology
Antibodies
Monoclonal
medicine.anatomical_structure
Female
Decidua Basalis
medicine.medical_specialty
Stromal cell
lcsh:QH471-489
Cell Survival
X-Linked Inhibitor of Apoptosis Protein
Biology
lcsh:Gynecology and obstetrics
Transforming Growth Factor beta1
03 medical and health sciences
Transforming Growth Factor beta2
Transforming Growth Factor beta3
Internal medicine
medicine
Animals
lcsh:RG1-991
030304 developmental biology
Research
Trophoblast
Transforming growth factor beta
medicine.disease
Rats
Reproductive Medicine
Gene Expression Regulation
biology.protein
Proto-Oncogene Proteins c-akt
Developmental Biology
Zdroj: Reproductive biology and endocrinology : RB&E
Reproductive Biology and Endocrinology, Vol 3, Iss 1, p 20 (2005)
ISSN: 1477-7827
Popis: BackgroundDuring early rat pregnancy, trophoblast of the tiny embryo joins with the endometrium and epithelial cells undergo apoptosis. Near the end of pregnancy, regression of the decidua basalis (DB) is also observed (from day 14 to 20). However, little is known about the intra-cellular and molecular mechanisms involved in apoptosis regulation in the uterus during pregnancy. The objective of the present study was to investigate the presence and the developmental expression of transforming growth factor-beta isoforms (TGF-beta well known differentiation factor) in the rat endometrium throughout pregnancy and its action in vitro using cultured endometrial stromal cells.MethodsIn vivo: Rats were killed at different days of pregnancy (days 2–20) and uteri removed to collect endometrial protein extracts or the uteri were fixed, embedded and sectioned for immunohistochemistry (IHC) and in situ cell death analyses using TdT-mediated dUTP nick end labeling (TUNEL). In vitro: Rats were ovariectomized and decidualization was induced using sex steroids. Endometrial stromal decidual cells were then collected and cultured.ResultsAn increase of apoptosis in the DB on days 14, 16 and 18 was observed. Cleaved caspase-3 was clearly detected during regression of the DB by Western analysis and immunofluorescence. Western analyses using endometrial protein extracts demonstrated that TGF-beta1, TGF-beta2 and TGF-beta3 were highly expressed at the time of DB regression (day 14). During early pregnancy, TGF-beta1 and -beta2 expressions raised at days 5.5 to 6.5. TGF-beta3 protein was not detected during early pregnancy. IHC analyses revealed that TGF-beta1 and -2 were found surrounding both epithelium (luminal and glandular) in the stroma compartment at the implantation site, and TGF-beta3 was mainly located surrounding endometrial epithelium in the stroma compartment. Smad2 phosphorylation was increased at the time of DB regression. In vitro studies using decidual endometrial stromal cells revealed that TGF-beta1 induced apoptosis and Smad2 phosphorylation. Moreover, TGF-beta1 reduced both Akt (a well known survival factor) phosphorylation and XIAP (X-linked inhibitor of apoptosis protein) expression in decidual endometrial stromal cells in vitro.ConclusionTaken together, these results suggest that TGF-beta isoforms are regulated differently during pregnancy and may have an important role in the control of apoptosis and cell survival at specific stages during pregnancy.
Databáze: OpenAIRE
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